論文 - 詳細
| RRC ID | 42891 |
|---|---|
| 著者 | Enosawa S, Miyashita T, Saito T, Omasa T, Matsumura T. |
| タイトル | The significant improvement of survival times and pathological parameters by bioartificial liver with recombinant HepG2 in porcine liver failure model. |
| ジャーナル | Cell Transplant |
| Abstract |
We developed a bioartificial liver (BAL) containing human hepatoblastoma cell line, HepG2, with the addition of ammonia removal activity by transfecting a glutamine synthetase (GS) gene and estimated the efficacy using pigs with ischemic liver failure. GS-HepG2 cells showed 15% ammonia removal activity of porcine hepatocytes, while unmodified HepG2 had no such activity. The established GS-HepG2 cells were grown in a circulatory flow bioreactor to 3.5-4.1 x 10(9) cells. Survival time of the animals treated with GS-HepG2 BAL was significantly prolonged compared to the cell-free control (14.52 +/- 5.24 h vs. 8.53 +/- 2.52 h) and the group treated with the BAL consisting of unmodified wild-type HepG2 (9.58 +/- 4.52 h). Comparison showed the cell-containing BAL groups to have significantly fewer incidences of increased brain pressure. Thus, the GS-HepG2 BAL treatment resulted in a significant improvement of survival time and pathological parameters in pigs with ischemic liver failure. |
| 巻・号 | 15(10) |
| ページ | 873-80 |
| 公開日 | 2006-1-1 |
| DOI | 10.3727/000000006783981350 |
| PMID | 17299991 |
| MeSH | Animals Bioreactors Cell Culture Techniques Cell Line, Tumor Cell Transplantation Cricetinae Disease Models, Animal Female Glutamate-Ammonia Ligase / genetics Glutamate-Ammonia Ligase / metabolism* Humans Liver Failure / metabolism Liver Failure / mortality Liver Failure / therapy* Liver, Artificial* Male Models, Biological Survival Rate Swine Transfection |
| IF | 3.341 |
| 引用数 | 23 |
| WOS 分野 | TRANSPLANTATION MEDICINE, RESEARCH & EXPERIMENTAL CELL & TISSUE ENGINEERING |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2(RCB0459) |