RRC ID 42909
著者 Nakatani S, Mano H, Im R, Shimizu J, Wada M.
タイトル Glucosamine regulates differentiation of a chondrogenic cell line, ATDC5.
ジャーナル Biol Pharm Bull
Abstract Osteoarthritis (OA) is a slowly progressing chronic joint disease. Glucosamine (GlcN) is a saccharide that is widely used to relieve symptoms associated with OA. However, the mechanism of the effects of GlcN on articular cartilage remains unclear. We studied the effects of GlcN and its analogues, including chitin derivatives included in health supplements containing GlcN, on a chondrogenic cell line, ATDC5. We examined the effects of these saccharides on the proliferation and differentiation of ATDC5 cells. Glucosamine analogues, such as N-acetyl glucosamine and chitobiose, did not affect the proliferation or differentiation of ATDC5 cells. While GlcN did not affect the proliferation of ATDC5 cells, it inhibited their differentiation. Next, we examined whether GlcN affects mineralization and glycosaminoglycan (GAG) production by ATDC5 cells. Mineralization was markedly inhibited by addition of GlcN to the cell culture medium. Moreover, GlcN induced the formation of sulfated GAG in ATDC5. We also analyzed the mRNA levels in ATDC5 cells. GlcN reduced the mRNA levels of Smad2, Smad4 and MGP. GlcN might inhibit expression of MGP mRNA and induce the production of chondroitin sulfate in ATDC5 cells. The mechanism by which GlcN inhibits mineralization may be by regulating the expression of mRNA for the Smad2 and Smad4 chondrogenic master genes.
巻・号 30(3)
ページ 433-8
公開日 2007-3-1
DOI 10.1248/bpb.30.433
PII JST.JSTAGE/bpb/30.433
PMID 17329833
MeSH Alkaline Phosphatase / antagonists & inhibitors Animals Calcification, Physiologic / drug effects Calcium-Binding Proteins / genetics Calcium-Binding Proteins / metabolism Cartilage / drug effects Cartilage / physiology Cell Differentiation / drug effects* Cell Line Cell Proliferation / drug effects Chondrocytes / cytology Chondrocytes / drug effects* Chondrocytes / metabolism Chondrogenesis / drug effects Chondrogenesis / genetics Extracellular Matrix Proteins / genetics Extracellular Matrix Proteins / metabolism Gene Expression / drug effects Gene Expression Profiling Glucosamine / analogs & derivatives Glucosamine / metabolism Glucosamine / pharmacology* Glycosaminoglycans / metabolism RNA, Messenger / genetics RNA, Messenger / metabolism Reverse Transcriptase Polymerase Chain Reaction Smad2 Protein / genetics Smad2 Protein / metabolism Smad4 Protein / genetics Smad4 Protein / metabolism Sulfates / metabolism
IF 1.863
引用数 14
WOS 分野 PHARMACOLOGY & PHARMACY
リソース情報
ヒト・動物細胞 ATDC5(RCB0565)