論文 - 詳細
| RRC ID | 42921 |
|---|---|
| 著者 | Nobeyama Y, Okochi-Takada E, Furuta J, Miyagi Y, Kikuchi K, Yamamoto A, Nakanishi Y, Nakagawa H, Ushijima T. |
| タイトル | Silencing of tissue factor pathway inhibitor-2 gene in malignant melanomas. |
| ジャーナル | Int J Cancer |
| Abstract |
To identify tumor-suppressor genes inactivated by aberrant methylation of promoter CpG islands (CGIs) in human malignant melanomas, genes upregulated by treatment of cells with a demethylating agent, 5-aza-2'-deoxycytidine (5-aza-dC), were searched for using oligonucleotide microarrays in melanoma cell lines, HMV-I, MeWo and WM-115. Seventy-nine known genes with CGIs were identified as being upregulated (>or=16-fold), and 18 of them had methylation of their putative promoter CGIs in 1 or more of 8 melanoma cell lines. Among the 18 genes, TFPI-2, which is involved in repression of the invasive potential of malignant melanomas, was further analyzed. Its expression was repressed in a melanoma cell line with its complete methylation, and was restored by 5-aza-dC treatment. It was unmethylated in cultured neonatal normal epidermal melanocyte, and was induced by ultraviolet B. In surgical melanoma specimens, TFPI-2 methylation was detected in 5 of 17 metastatic site specimens (29%), while it was not detected in 20 primary site specimens (0%) (p=0.009). By immunohistochemistry, the 5 specimens with promoter methylation lacked immunoreactivity for TFPI-2. The results showed that TFPI-2 is silenced in human malignant melanomas by methylation of its promoter CGI and suggested that its silencing is involved in melanoma metastasis. |
| 巻・号 | 121(2) |
| ページ | 301-7 |
| 公開日 | 2007-7-15 |
| DOI | 10.1002/ijc.22637 |
| PMID | 17372906 |
| MeSH | Azacitidine / analogs & derivatives Azacitidine / pharmacology Cell Line, Tumor Cells, Cultured CpG Islands DNA Methylation / drug effects Decitabine Gene Expression Regulation / radiation effects Gene Silencing* Glycoproteins / genetics* Glycoproteins / metabolism Humans Immunohistochemistry Melanoma / genetics* Melanoma / metabolism Melanoma / pathology Neoplasm Metastasis Oligonucleotide Array Sequence Analysis Promoter Regions, Genetic / genetics Reverse Transcriptase Polymerase Chain Reaction Ultraviolet Rays |
| IF | 5.145 |
| 引用数 | 46 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MMAc(RCB0808) COLO 679(RCB0989) |