| RRC ID |
42939
|
| 著者 |
Dadsetan M, Szatkowski JP, Yaszemski MJ, Lu L.
|
| タイトル |
Characterization of photo-cross-linked oligo[poly(ethylene glycol) fumarate] hydrogels for cartilage tissue engineering.
|
| ジャーナル |
Biomacromolecules
|
| Abstract |
Photo-cross-linkable oligo[poly(ethylene glycol) fumarate] (OPF) hydrogels have been developed for use in tissue engineering applications. We demonstrated that compressive modulus of these hydrogels increased with increasing polymer concentration, and hydrogels with different mechanical properties were formed by altering the ratio of cross-linker/polymer in precursor solution. Conversely, swelling of hydrogels decreased with increasing polymer concentration and cross-linker/polymer ratio. These hydrogels are degradable and degradation rates vary with the change in cross-linking level. Chondrocyte attachment was quantified as a method for evaluating adhesion of cells to the hydrogels. These data revealed that cross-linking density affects cell behavior on the hydrogel surfaces. Cell attachment was greater on the samples with increased cross-linking density. Chondrocytes on these samples exhibited spread morphology with distinct actin stress fibers, whereas they maintained their rounded morphology on the samples with lower cross-linking density. Moreover, chondrocytes were photoencapsulated within various hydrogel networks. Our results revealed that cells encapsulated within 2-mm thick OPF hydrogel disks remained viable throughout the 3-week culture period, with no difference in viability across the thickness of hydrogels. Photoencapsulated chondrocytes expressed the mRNA of type II collagen and produced cartilaginous matrix within the hydrogel constructs after three weeks. These findings suggest that photo-cross-linkable OPF hydrogels may be useful for cartilage tissue engineering and cell delivery applications.
|
| 巻・号 |
8(5)
|
| ページ |
1702-9
|
| 公開日 |
2007-5-1
|
| DOI |
10.1021/bm070052h
|
| PMID |
17419584
|
| MeSH |
Animals
Cartilage / cytology
Cartilage / growth & development*
Cell Adhesion
Cell Line
Cell Survival
Chondrocytes / chemistry
Chondrocytes / cytology*
Chondrocytes / metabolism
Collagen Type II / analysis
Collagen Type II / genetics
Collagen Type II / metabolism
Hydrogels / chemical synthesis
Hydrogels / chemistry*
Mice
Photochemistry
Polyesters / chemical synthesis
Polyesters / radiation effects*
Polyethylene Glycols / chemical synthesis
Polyethylene Glycols / radiation effects*
RNA, Messenger / analysis
RNA, Messenger / metabolism
Surface Properties
Tissue Engineering*
Ultraviolet Rays
|
| IF |
6.092
|
| 引用数 |
63
|
|
WOS 分野
|
POLYMER SCIENCE
CHEMISTRY, ORGANIC
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
