Reference - Detail
| RRC ID | 42964 |
|---|---|
| Author | Takeuchi S, Mukai N, Tateishi T, Miyakawa S. |
| Title | Production of sex steroid hormones from DHEA in articular chondrocyte of rats. |
| Journal | Am J Physiol Endocrinol Metab |
| Abstract |
Dehydroepiandrosterone (DHEA), a precursor of sex steroid hormones, is synthesized by cholesterol side-chain cleavage cytochrome P-450 and 17alpha-hydroxylase cytochrome P-450 mainly from cholesterol and converted to testosterone and estrogen by 3beta-hydroxysteroid dehydrogenase (3beta-HSD), 17beta-HSD, and aromatase cytochrome P-450. Although sex steroid hormones have important effects in the protection of articular cartilage, it is unclear whether articular cartilage has a local steroidogenic enzymatic machinery capable of metabolizing DHEA. This study was aimed to clarify whether steroidogenesis-related enzymes are expressed in articular chondrocytes, whether expression levels are changed by DHEA, and whether articular chondrocytes are capable of synthesizing sex steroid hormones from DHEA. Articular chondrocytes isolated from adult rats were cultured with DHEA for 3 days. All of the mRNA expressions of steroidogenesis-related enzymes were detected in cultured articular chondrocytes of rats, but the mRNA expression levels of testosterone and estradiol in cultured media increased after the addition of DHEA. These findings provided the first evidence that articular chondrocytes expressed steroidogenesis-related enzyme genes and that they are capable of locally synthesizing sex steroid hormones locally from DHEA. |
| Volume | 293(1) |
| Pages | E410-5 |
| Published | 2007-7-1 |
| DOI | 10.1152/ajpendo.00042.2007 |
| PII | 00042.2007 |
| PMID | 17473054 |
| MeSH | 17-Hydroxysteroid Dehydrogenases / genetics 17-Hydroxysteroid Dehydrogenases / metabolism 3-Hydroxysteroid Dehydrogenases / genetics 3-Hydroxysteroid Dehydrogenases / metabolism Animals Aromatase / genetics Aromatase / metabolism Cartilage, Articular / cytology Cartilage, Articular / enzymology* Cartilage, Articular / metabolism* Cells, Cultured Cholesterol Side-Chain Cleavage Enzyme / genetics Cholesterol Side-Chain Cleavage Enzyme / metabolism Chondrocytes / drug effects Chondrocytes / enzymology* Chondrocytes / metabolism* Collagen Type II / genetics Collagen Type II / metabolism Dehydroepiandrosterone / metabolism* Dehydroepiandrosterone / pharmacology Dose-Response Relationship, Drug Female Gene Expression Regulation, Enzymologic / drug effects Gonadal Steroid Hormones / biosynthesis* RNA, Messenger / metabolism Rats Rats, Sprague-Dawley |
| IF | 3.469 |
| Times Cited | 17 |
| WOS Category | ENDOCRINOLOGY & METABOLISM PHYSIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Wikipedia |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | ATDC5(RCB0565) |