RRC ID 42968
Author Yamada P, Isoda H, Han JK, Talorete TP, Abe Y.
Title Inhibitory effect of fulvic acid extracted from Canadian sphagnum peat on chemical mediator release by RBL-2H3 and KU812 cells.
Journal Biosci Biotechnol Biochem
Abstract Fulvic acid (FA) was extracted and purified from Canadian Sphagnum peat (CP-FA) and characterized by using an element analysis meter, Fourier transform infrared (FT-IR) spectroscopy, electron spin resonance (ESR) spectroscopy, and (13)C-nuclear magnetic resonance ((13)C-NMR) spectroscopy. To investigate the antiallergic effect of CP-FA, we incubated rat basophilic leukemia (RBL-2H3) cells with 0.001-10.0 microg/ml of CP-FA and determined the beta-hexosaminidase release inhibition at different response stages. The intracellular calcium [Ca(2+)](i) level was also determined by using Fluo 3-AM, a calcium-specific fluorescent probe, and the cytotoxicity of CP-FA was determined by the 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. The results revealed that RBL-2H3 cells incubated for 48 h with 0.001-10.0 microg/ml of CP-FA did not show any decreased viability. CP-FA inhibited the beta-hexosaminidase release by IgE-sensitized, antigen-stimulated RBL-2H3 cells at the antigen-antibody binding stage and the antibody-receptor binding stage. CP-FA also inhibited histamine release from A23187 plus PMA- or compound 48/80-stimulated KU812 cells. Furthermore, there was a decrease in the intracellular [Ca(2+)](i) level in IgE-sensitized cells incubated with CP-FA and stimulated with antigen. Our results show that CP-FA may be useful for the treatment or prevention of allergic diseases.
Volume 71(5)
Pages 1294-305
Published 2007-5-1
DOI 10.1271/bbb.60702
PII JST.JSTAGE/bbb/60702
PMID 17485833
MeSH Animals Anti-Allergic Agents / pharmacology* Antigen-Antibody Complex / immunology Antigens / immunology Basophils / metabolism* Benzopyrans / pharmacology* Calcimycin / pharmacology Calcium / analysis Cell Line, Tumor Dose-Response Relationship, Drug Fluorescent Dyes Formazans / metabolism Histamine Release / drug effects* Humans Immunoglobulin E / pharmacology Ionophores / pharmacology Leukemia, Basophilic, Acute Leukemia, Experimental Leukemia, Myelogenous, Chronic, BCR-ABL Positive Nuclear Magnetic Resonance, Biomolecular Plant Extracts / pharmacology* Rats Spectroscopy, Fourier Transform Infrared Sphagnopsida / chemistry* Tetradecanoylphorbol Acetate / pharmacology Tetrazolium Salts / metabolism beta-N-Acetylhexosaminidases / antagonists & inhibitors p-Methoxy-N-methylphenethylamine / pharmacology
IF 1.516
Times Cited 38
Human and Animal Cells RBL 2H3(RCB2782)