| RRC ID |
42968
|
| 著者 |
Yamada P, Isoda H, Han JK, Talorete TP, Abe Y.
|
| タイトル |
Inhibitory effect of fulvic acid extracted from Canadian sphagnum peat on chemical mediator release by RBL-2H3 and KU812 cells.
|
| ジャーナル |
Biosci Biotechnol Biochem
|
| Abstract |
Fulvic acid (FA) was extracted and purified from Canadian Sphagnum peat (CP-FA) and characterized by using an element analysis meter, Fourier transform infrared (FT-IR) spectroscopy, electron spin resonance (ESR) spectroscopy, and (13)C-nuclear magnetic resonance ((13)C-NMR) spectroscopy. To investigate the antiallergic effect of CP-FA, we incubated rat basophilic leukemia (RBL-2H3) cells with 0.001-10.0 microg/ml of CP-FA and determined the beta-hexosaminidase release inhibition at different response stages. The intracellular calcium [Ca(2+)](i) level was also determined by using Fluo 3-AM, a calcium-specific fluorescent probe, and the cytotoxicity of CP-FA was determined by the 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. The results revealed that RBL-2H3 cells incubated for 48 h with 0.001-10.0 microg/ml of CP-FA did not show any decreased viability. CP-FA inhibited the beta-hexosaminidase release by IgE-sensitized, antigen-stimulated RBL-2H3 cells at the antigen-antibody binding stage and the antibody-receptor binding stage. CP-FA also inhibited histamine release from A23187 plus PMA- or compound 48/80-stimulated KU812 cells. Furthermore, there was a decrease in the intracellular [Ca(2+)](i) level in IgE-sensitized cells incubated with CP-FA and stimulated with antigen. Our results show that CP-FA may be useful for the treatment or prevention of allergic diseases.
|
| 巻・号 |
71(5)
|
| ページ |
1294-305
|
| 公開日 |
2007-5-1
|
| DOI |
10.1271/bbb.60702
|
| PII |
JST.JSTAGE/bbb/60702
|
| PMID |
17485833
|
| MeSH |
Animals
Anti-Allergic Agents / pharmacology*
Antigen-Antibody Complex / immunology
Antigens / immunology
Basophils / metabolism*
Benzopyrans / pharmacology*
Calcimycin / pharmacology
Calcium / analysis
Cell Line, Tumor
Dose-Response Relationship, Drug
Fluorescent Dyes
Formazans / metabolism
Histamine Release / drug effects*
Humans
Immunoglobulin E / pharmacology
Ionophores / pharmacology
Leukemia, Basophilic, Acute
Leukemia, Experimental
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Nuclear Magnetic Resonance, Biomolecular
Plant Extracts / pharmacology*
Rats
Spectroscopy, Fourier Transform Infrared
Sphagnopsida / chemistry*
Tetradecanoylphorbol Acetate / pharmacology
Tetrazolium Salts / metabolism
beta-N-Acetylhexosaminidases / antagonists & inhibitors
p-Methoxy-N-methylphenethylamine / pharmacology
|
| IF |
1.516
|
| 引用数 |
38
|
|
WOS 分野
|
FOOD SCIENCE & TECHNOLOGY
CHEMISTRY, APPLIED
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
RBL 2H3(RCB2782) |
