| RRC ID |
4297
|
| 著者 |
Simon DJ, Madison JM, Conery AL, Thompson-Peer KL, Soskis M, Ruvkun GB, Kaplan JM, Kim JK.
|
| タイトル |
The microRNA miR-1 regulates a MEF-2-dependent retrograde signal at neuromuscular junctions.
|
| ジャーナル |
Cell
|
| Abstract |
We show that miR-1, a conserved muscle-specific microRNA, regulates aspects of both pre- and postsynaptic function at C. elegans neuromuscular junctions. miR-1 regulates the expression level of two nicotinic acetylcholine receptor (nAChR) subunits (UNC-29 and UNC-63), thereby altering muscle sensitivity to acetylcholine (ACh). miR-1 also regulates the muscle transcription factor MEF-2, which results in altered presynaptic ACh secretion, suggesting that MEF-2 activity in muscles controls a retrograde signal. The effect of the MEF-2-dependent retrograde signal on secretion is mediated by the synaptic vesicle protein RAB-3. Finally, acute activation of levamisole-sensitive nAChRs stimulates MEF-2-dependent transcriptional responses and induces the MEF-2-dependent retrograde signal. We propose that miR-1 refines synaptic function by coupling changes in muscle activity to changes in presynaptic function.
|
| 巻・号 |
133(5)
|
| ページ |
903-15
|
| 公開日 |
2008-5-30
|
| DOI |
10.1016/j.cell.2008.04.035
|
| PII |
S0092-8674(08)00609-0
|
| PMID |
18510933
|
| PMC |
PMC2553566
|
| MeSH |
Animals
Caenorhabditis elegans / metabolism*
Caenorhabditis elegans Proteins / metabolism*
Levamisole / pharmacology
MicroRNAs / genetics
MicroRNAs / metabolism*
Mutation
Neuromuscular Junction / metabolism*
Nicotinic Agonists / metabolism
Receptors, Nicotinic / metabolism
Signal Transduction*
Transcription Factors / metabolism*
Transcription, Genetic
rab3 GTP-Binding Proteins / metabolism
|
| IF |
38.637
|
| 引用数 |
135
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| 線虫 |
tm1635 |
