RRC ID 43022
Author Islam S, Hassan F, Tumurkhuu G, Dagvadorj J, Koide N, Naiki Y, Mori I, Yoshida T, Yokochi T.
Title Bacterial lipopolysaccharide induces osteoclast formation in RAW 264.7 macrophage cells.
Journal Biochem Biophys Res Commun
Abstract Lipopolysaccharide (LPS) is a potent bone resorbing factor. The effect of LPS on osteoclast formation was examined by using murine RAW 264.7 macrophage cells. LPS-induced the formation of multinucleated giant cells (MGC) in RAW 264.7 cells 3 days after the exposure. MGCs were positive for tartrate-resistant acid phosphatase (TRAP) activity. Further, MGC formed resorption pits on calcium-phosphate thin film that is a substrate for osteoclasts. Therefore, LPS was suggested to induce osteoclast formation in RAW 264.7 cells. LPS-induced osteoclast formation was abolished by anti-tumor necrosis factor (TNF)-alpha antibody, but not antibodies to macrophage-colony stimulating factor (M-CSF) and receptor activator of nuclear factor (NF)-kappaB ligand (RANKL). TNF-alpha might play a critical role in LPS-induced osteoclast formation in RAW 264.7 cells. Inhibitors of NF-kappaB and stress activated protein kinase (SAPK/JNK) prevented the LPS-induced osteoclast formation. The detailed mechanism of LPS-induced osteoclast formation is discussed.
Volume 360(2)
Pages 346-51
Published 2007-8-24
DOI 10.1016/j.bbrc.2007.06.023
PII S0006-291X(07)01257-0
PMID 17597583
MeSH Animals Bone Resorption / metabolism* Bone Resorption / pathology Cell Differentiation / drug effects Cell Differentiation / physiology Cell Line Escherichia coli / metabolism* Lipopolysaccharides / administration & dosage Lipopolysaccharides / metabolism* Macrophages / drug effects Macrophages / metabolism* Macrophages / pathology Mice Osteoclasts / drug effects* Osteoclasts / pathology*
IF 2.985
Times Cited 82
Human and Animal Cells