RRC ID 43038
Author Takezawa T, Takeuchi T, Nitani A, Takayama Y, Kino-Oka M, Taya M, Enosawa S.
Title Collagen vitrigel membrane useful for paracrine assays in vitro and drug delivery systems in vivo.
Journal J Biotechnol
Abstract We previously succeeded in converting a soft and turbid disk of type-I collagen gel into a strong and transparent vitrigel membrane utilizing a concept for the vitrification of heat-denatured proteins and have demonstrated its protein-permeability and advantage as a scaffold for reconstructing crosstalk models between two different cell types. In this study, we observed the nano-structure of the type-I collagen vitrigel membrane and verified its utility for paracrine assays in vitro and drug delivery systems in vivo. Scanning electron microscopic observation revealed that the vitrigel membrane was a dense network architecture of typical type-I collagen fibrils. In the crosstalk model between PC-12 pheochromocytoma cells and L929 fibroblasts, nerve growth factor (NGF) secreted from L929 cells passed through the collagen vitrigel membrane and induced the neurite outgrowth of PC-12 cells by its paracrine effect. Also, the collagen vitrigel membrane containing vascular endothelial growth factor (VEGF) showed sustained-release of VEGF in vitro and its subcutaneous transplantation into a rat resulted in remarkable angiogenesis. These data suggest that the collagen vitrigel membrane is useful for paracrine assays in vitro and drug delivery systems in vivo.
Volume 131(1)
Pages 76-83
Published 2007-8-1
DOI 10.1016/j.jbiotec.2007.05.033
PII S0168-1656(07)00385-9
PMID 17624459
MeSH Animals Biological Assay / instrumentation* Cell Differentiation Collagen Type I / metabolism* Collagen Type I / ultrastructure Drug Delivery Systems / instrumentation* Male Membranes, Artificial* Nanostructures Neovascularization, Physiologic Nerve Growth Factor / metabolism PC12 Cells Paracrine Communication* Permeability Prostheses and Implants Rats Rats, Wistar Vascular Endothelial Growth Factor A / metabolism
IF 3.163
Times Cited 42
Human and Animal Cells