Reference - Detail
| RRC ID | 43038 |
|---|---|
| Author | Takezawa T, Takeuchi T, Nitani A, Takayama Y, Kino-Oka M, Taya M, Enosawa S. |
| Title | Collagen vitrigel membrane useful for paracrine assays in vitro and drug delivery systems in vivo. |
| Journal | J Biotechnol |
| Abstract |
We previously succeeded in converting a soft and turbid disk of type-I collagen gel into a strong and transparent vitrigel membrane utilizing a concept for the vitrification of heat-denatured proteins and have demonstrated its protein-permeability and advantage as a scaffold for reconstructing crosstalk models between two different cell types. In this study, we observed the nano-structure of the type-I collagen vitrigel membrane and verified its utility for paracrine assays in vitro and drug delivery systems in vivo. Scanning electron microscopic observation revealed that the vitrigel membrane was a dense network architecture of typical type-I collagen fibrils. In the crosstalk model between PC-12 pheochromocytoma cells and L929 fibroblasts, nerve growth factor (NGF) secreted from L929 cells passed through the collagen vitrigel membrane and induced the neurite outgrowth of PC-12 cells by its paracrine effect. Also, the collagen vitrigel membrane containing vascular endothelial growth factor (VEGF) showed sustained-release of VEGF in vitro and its subcutaneous transplantation into a rat resulted in remarkable angiogenesis. These data suggest that the collagen vitrigel membrane is useful for paracrine assays in vitro and drug delivery systems in vivo. |
| Volume | 131(1) |
| Pages | 76-83 |
| Published | 2007-8-1 |
| DOI | 10.1016/j.jbiotec.2007.05.033 |
| PII | S0168-1656(07)00385-9 |
| PMID | 17624459 |
| MeSH | Animals Biological Assay / instrumentation* Cell Differentiation Collagen Type I / metabolism* Collagen Type I / ultrastructure Drug Delivery Systems / instrumentation* Male Membranes, Artificial* Nanostructures Neovascularization, Physiologic Nerve Growth Factor / metabolism PC12 Cells Paracrine Communication* Permeability Prostheses and Implants Rats Rats, Wistar Vascular Endothelial Growth Factor A / metabolism |
| IF | 3.503 |
| Times Cited | 42 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 9 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | PC-12(RCB0009) |