| RRC ID |
43038
|
| 著者 |
Takezawa T, Takeuchi T, Nitani A, Takayama Y, Kino-Oka M, Taya M, Enosawa S.
|
| タイトル |
Collagen vitrigel membrane useful for paracrine assays in vitro and drug delivery systems in vivo.
|
| ジャーナル |
J Biotechnol
|
| Abstract |
We previously succeeded in converting a soft and turbid disk of type-I collagen gel into a strong and transparent vitrigel membrane utilizing a concept for the vitrification of heat-denatured proteins and have demonstrated its protein-permeability and advantage as a scaffold for reconstructing crosstalk models between two different cell types. In this study, we observed the nano-structure of the type-I collagen vitrigel membrane and verified its utility for paracrine assays in vitro and drug delivery systems in vivo. Scanning electron microscopic observation revealed that the vitrigel membrane was a dense network architecture of typical type-I collagen fibrils. In the crosstalk model between PC-12 pheochromocytoma cells and L929 fibroblasts, nerve growth factor (NGF) secreted from L929 cells passed through the collagen vitrigel membrane and induced the neurite outgrowth of PC-12 cells by its paracrine effect. Also, the collagen vitrigel membrane containing vascular endothelial growth factor (VEGF) showed sustained-release of VEGF in vitro and its subcutaneous transplantation into a rat resulted in remarkable angiogenesis. These data suggest that the collagen vitrigel membrane is useful for paracrine assays in vitro and drug delivery systems in vivo.
|
| 巻・号 |
131(1)
|
| ページ |
76-83
|
| 公開日 |
2007-8-1
|
| DOI |
10.1016/j.jbiotec.2007.05.033
|
| PII |
S0168-1656(07)00385-9
|
| PMID |
17624459
|
| MeSH |
Animals
Biological Assay / instrumentation*
Cell Differentiation
Collagen Type I / metabolism*
Collagen Type I / ultrastructure
Drug Delivery Systems / instrumentation*
Male
Membranes, Artificial*
Nanostructures
Neovascularization, Physiologic
Nerve Growth Factor / metabolism
PC12 Cells
Paracrine Communication*
Permeability
Prostheses and Implants
Rats
Rats, Wistar
Vascular Endothelial Growth Factor A / metabolism
|
| IF |
3.503
|
| 引用数 |
42
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
PC-12(RCB0009) |
