論文 - 詳細
| RRC ID | 43047 |
|---|---|
| 著者 | Matsuoka TA, Kaneto H, Stein R, Miyatsuka T, Kawamori D, Henderson E, Kojima I, Matsuhisa M, Hori M, Yamasaki Y. |
| タイトル | MafA regulates expression of genes important to islet beta-cell function. |
| ジャーナル | Mol Endocrinol |
| Abstract |
Insulin transcription factor MafA is unique in being exclusively expressed at the secondary and principal phase of insulin-expressing cell production during pancreas organogenesis and is the only transcriptional activator present exclusively in islet beta-cells. Here we show that ectopic expression of MafA is sufficient to induce a small amount of endogenous insulin expression in a variety of non-beta-cell lines. Insulin mRNA and protein expression was induced to a much higher level when MafA was provided with two other key insulin activators, pancreatic and duodenal homeobox (PDX-1) and BETA2. Potentiation by PDX-1 and BETA2 was entirely dependent upon MafA, and MafA binding to the insulin enhancer region was increased by PDX-1 and BETA2. Treatment with activin A and hepatocyte growth factor induced even larger amounts of insulin in AR42J pancreatic acinar cells, compared with other non-beta endodermal cells. The combination of PDX-1, BETA2, and MafA also induced the expression of other important regulators of islet beta-cell activity. These results support a critical role of MafA in islet beta-cell function. |
| 巻・号 | 21(11) |
| ページ | 2764-74 |
| 公開日 | 2007-11-1 |
| DOI | 10.1210/me.2007-0028 |
| PII | me.2007-0028 |
| PMID | 17636040 |
| MeSH | Animals Cell Nucleus / metabolism Gene Expression Regulation* Glucose Transporter Type 2 / metabolism HeLa Cells Humans Insulin / metabolism Insulin-Secreting Cells / metabolism* Islets of Langerhans / metabolism* Maf Transcription Factors, Large / metabolism Maf Transcription Factors, Large / physiology* Mice Models, Biological Protein Binding RNA, Messenger / metabolism Transcriptional Activation |
| IF | 3.628 |
| 引用数 | 71 |
| WOS 分野 | ENDOCRINOLOGY & METABOLISM |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | IEC 6(RCB0993) |