論文 - 詳細
| RRC ID | 43077 |
|---|---|
| 著者 | Tachibana S, Hirano M, Hirata T, Matsuo M, Ikeda I, Ueda K, Sato R. |
| タイトル | Cholesterol and plant sterol efflux from cultured intestinal epithelial cells is mediated by ATP-binding cassette transporters. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
In this study we analyzed functions of ATP-binding cassette (ABC) transporters involved in sterol transport from Caco-2 cells. Treatment with a synthetic liver x receptor ligand elevated both mRNA and protein levels of ABCG5, G8, and ABCA1. The ligand stimulated cholesterol efflux, suggesting that ABC transporters are involved in it. To identify the acceptors of cholesterol, potential molecules such as apolipoprotein A-I, glycocholic acid, phosphatidylcholine, and bile acid micelles were added to the medium. Apo A-I, a known acceptor of cholesterol transported by ABCA1, elevated cholesterol efflux on the basal side, whereas the others raised cholesterol efflux on the apical side. Moreover, bile acid micelles preferentially augmented plant sterol efflux rather than cholesterol. Finally, in HEK293 cells stably expressing ABCG5/G8, bile acid micelle-mediated sterol efflux was significantly accelerated. These results indicate that ABCG5/G8, unlike ABCA1, together with bile acids should participate in sterol efflux on the apical surface of Caco-2 cells. |
| 巻・号 | 71(8) |
| ページ | 1886-95 |
| 公開日 | 2007-8-1 |
| DOI | 10.1271/bbb.70109 |
| PII | JST.JSTAGE/bbb/70109 |
| PMID | 17690481 |
| MeSH | ATP Binding Cassette Transporter 1 ATP Binding Cassette Transporter, Subfamily G, Member 5 ATP Binding Cassette Transporter, Subfamily G, Member 8 ATP-Binding Cassette Transporters / metabolism* Bile Acids and Salts / metabolism Biological Transport Caco-2 Cells Cell Line Cholesterol / metabolism* DNA-Binding Proteins / agonists Epithelial Cells / metabolism* Humans Intestines / cytology Lipoproteins Liver X Receptors Orphan Nuclear Receptors Phytosterols / metabolism* Receptors, Cytoplasmic and Nuclear / agonists |
| IF | 1.516 |
| 引用数 | 23 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | CACO-2(RCB0988) |