RRC ID 43082
Author Kanno T, Kawanishi M, Takamura-Enya T, Arlt VM, Phillips DH, Yagi T.
Title DNA adduct formation in human hepatoma cells treated with 3-nitrobenzanthrone: analysis by the (32)P-postlabeling method.
Journal Mutat. Res.
Abstract 3-Nitrobenzanthrone (3-nitro-7H-benz[d,e]anthracen-7-one, 3-NBA) is a powerful mutagen and a suspected human carcinogen existing in diesel exhaust and airborne particulates. Recently, one of the major presumed metabolites of 3-NBA, 3-aminobenzanthrone (3-ABA), was detected in human urine samples. Here we analyzed DNA adducts formed in 3-NBA-exposed human hepatoma HepG2 cells by a (32)P-postlabeling/thin layer chromatography (TLC) method and a (32)P-postlabeling/polyacrylamide gel electrophoresis (PAGE) method. With HepG2 cells exposed to 3-NBA (0.36-36.4 microM) for 3h, we obtained three spots or bands corresponding to adducted nucleotides. Two were assigned as 2-(2'-deoxyadenosin-N(6)-yl)-3-aminobenzanthrone-3'-phosphate (dA3'p-N(6)-C2-ABA) and 2-(2'-deoxyguanosin-N(2)-yl)-3-aminobenzanthrone-3'-phosphate (dG3'p-N(2)-C2-ABA), with identical mobilities to those of synthetic standards on PAGE analysis. The chemical structure of the substance corresponding to the other spot or band could not be identified. Quantitative analyses revealed that the major adduct was dA3'p-N(6)-C2-ABA and its relative adduct labeling (RAL) value at 36.4 microM of 3-NBA was 200.8+/-86.1/10(8)nucleotide.
Volume 634(1-2)
Pages 184-91
Published 2007-12-1
DOI 10.1016/j.mrgentox.2007.07.002
PII S1383-5718(07)00217-3
PMID 17702645
MeSH Benz(a)Anthracenes / pharmacology* Carcinoma, Hepatocellular / genetics* Cell Line, Tumor Chromatography, Thin Layer / methods DNA Adducts / metabolism* Electrophoresis, Polyacrylamide Gel Humans Liver Neoplasms / genetics* Phosphorus Radioisotopes
IF 2.398
Times Cited 8
WOS Category BIOTECHNOLOGY & APPLIED MICROBIOLOGY TOXICOLOGY GENETICS & HEREDITY
Resource
Human and Animal Cells