RRC ID 43086
著者 Sakai S, Hashimoto I, Kawakami K.
タイトル Production of cell-enclosing hollow-core agarose microcapsules via jetting in water-immiscible liquid paraffin and formation of embryoid body-like spherical tissues from mouse ES cells enclosed within these microcapsules.
ジャーナル Biotechnol Bioeng
Abstract We developed agarose microcapsules with a single hollow core templated by alginate microparticles using a jet-technique. We extruded an agarose aqueous solution containing suspended alginate microparticles into a coflowing stream of liquid paraffin and controlled the diameter of the agarose microparticles by changing the flow rate of the liquid paraffin. Subsequent degradation of the inner alginate microparticles using alginate lyase resulted in the hollow-core structure. We successfully obtained agarose microcapsules with 20-50 microm of agarose gel layer thickness and hollow cores ranging in diameter from ca. 50 to 450 microm. Using alginate microparticles of ca. 150 microm in diameter and enclosing feline kidney cells, we were able to create cell-enclosing agarose microcapsules with a hollow core of ca. 150 microm in diameter. The cells in these microcapsules grew much faster than those in alginate microparticles. In addition, we enclosed mouse embryonic stem cells in agarose microcapsules. The embryonic stem cells began to self-aggregate in the core just after encapsulation, and subsequently grew and formed embryoid body-like spherical tissues in the hollow core of the microcapsules. These results show that our novel microcapsule production technique and the resultant microcapsules have potential for tissue engineering, cell therapy and biopharmaceutical applications.
巻・号 99(1)
ページ 235-43
公開日 2008-1-1
DOI 10.1002/bit.21624
PMID 17705234
MeSH Animals Capsules Cell Culture Techniques / methods* Cells, Cultured Embryo, Mammalian / cytology* Embryonic Development / physiology* Embryonic Stem Cells / cytology* Embryonic Stem Cells / physiology* Emulsions / chemistry Mice Paraffin / chemistry Porosity Pressure Sepharose / chemistry* Tissue Engineering / methods* Water / chemistry
IF 4.002
引用数 46
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
リソース情報
ヒト・動物細胞 H-1(AES0001)