論文 - 詳細
| RRC ID | 43103 |
|---|---|
| 著者 | Kajiwara T, Yasuda T, Matsuura E. |
| タイトル | Intracellular trafficking of beta2-glycoprotein I complexes with lipid vesicles in macrophages: implications on the development of antiphospholipid syndrome. |
| ジャーナル | J Autoimmun |
| Abstract |
Beta(2)-glycoprotein I (beta(2)GPI) is known as a major autoantigen for antiphospholipid antibodies. Our recent data show that binding of beta(2)GPI to oxidized low-density lipoprotein (oxLDL) or to liposomes containing anionic phospholipid(s) may facilitate the presentation of beta(2)GPI's epitope by macrophages/dendritic cells to autoreactive T cells. In the present study, we investigated intracellular trafficking of beta(2)GPI and its complexes with oxLDL or liposomes containing phosphatidylserine (PS-liposomes) in mouse macrophage-like J774 cells. A relatively small amount of non-complexed beta(2)GPI was taken up and stagnated in the late endosome after incubating for 16h. In contrast, beta(2)GPI complexes with oxLDL or PS-liposomes were transported into the lysosome. In the presence of the IgG anti-beta(2)GPI autoantibody, WB-CAL-1, beta(2)GPI/oxLDL complexes were rapidly incorporated into intracellular space and were finally localized in the lysosome. Interestingly, in vitro pulses by beta(2)GPI/oxLDL complexes together with WB-CAL-1 led to the expression of membranous CD36 as well as Fcgamma type I receptors (FcgammaRI). These observations suggest that IgG immune complexes of beta(2)GPI/oxLDL provide not only FcgammaRI- but also scavenger receptor-mediated uptake of beta(2)GPI/oxLDL complexes by macrophages. Thus, beta(2)GPI/oxLDL complexes as a major atherogenic autoantigen and IgG anti-beta(2)GPI autoantibodies may facilitate antigen presentation and foam cell formation in antiphospholipid syndrome. |
| 巻・号 | 29(2-3) |
| ページ | 164-73 |
| 公開日 | 2007-1-1 |
| DOI | 10.1016/j.jaut.2007.07.003 |
| PII | S0896-8411(07)00082-0 |
| PMID | 17826950 |
| MeSH | Animals Antibodies, Antiphospholipid / immunology* Antibodies, Antiphospholipid / metabolism Antigen-Antibody Complex / metabolism Antiphospholipid Syndrome / immunology* Autoantibodies / immunology Autoantigens / immunology Cell Line Endosomes / immunology Endosomes / metabolism Lipoproteins, LDL / immunology Lipoproteins, LDL / metabolism* Liposomes / immunology Liposomes / metabolism Lysosomes / immunology Lysosomes / metabolism Macrophages / cytology Macrophages / immunology* Mice Receptors, Scavenger / immunology Receptors, Scavenger / metabolism beta 2-Glycoprotein I / immunology* beta 2-Glycoprotein I / isolation & purification beta 2-Glycoprotein I / metabolism* |
| IF | 6.658 |
| 引用数 | 37 |
| WOS 分野 | IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | |