RRC ID 43105
Author Rahioui I, Laugier C, Balmand S, Da Silva P, Rahbe Y, Gressent F.
Title Toxicity, binding and internalization of the pea-A1b entomotoxin in Sf9 cells.
Journal Biochimie
Abstract PA1b (Pea Albumin 1b) is a peptide toxin lethal for certain insects. This paper shows that the cultured insect cells Sf9 are sensitive to the toxin and display a high-affinity binding site for PA1b. Mammalian cells are not sensitive and no binding activity was detected. Signs of apoptosis of the Sf9 cells were observed in response to the toxin. The use of this cellular model also demonstrated that PA1b was internalized in the cells, via the binding site, raising the new question of the role of this toxin within the cell, and of the mechanisms leading to cell death.
Volume 89(12)
Pages 1539-43
Published 2007-12
DOI 10.1016/j.biochi.2007.07.021
PII S0300-9084(07)00197-6
PMID 17845830
MeSH Albumins / chemistry* Animals Apoptosis / drug effects Binding Sites Cell Culture Techniques Cell Survival / drug effects Cells, Cultured Endotoxins / chemistry* Endotoxins / isolation & purification Endotoxins / pharmacology Iodine Radioisotopes / metabolism Kinetics Molecular Weight Peas / metabolism* Plant Proteins / chemistry Protein Binding Protein Isoforms / chemistry Spodoptera / cytology* Spodoptera / metabolism* Spodoptera / ultrastructure Temperature Time Factors
IF 3.188
Times Cited 10
Human and Animal Cells