| RRC ID |
43105
|
| 著者 |
Rahioui I, Laugier C, Balmand S, Da Silva P, Rahbe Y, Gressent F.
|
| タイトル |
Toxicity, binding and internalization of the pea-A1b entomotoxin in Sf9 cells.
|
| ジャーナル |
Biochimie
|
| Abstract |
PA1b (Pea Albumin 1b) is a peptide toxin lethal for certain insects. This paper shows that the cultured insect cells Sf9 are sensitive to the toxin and display a high-affinity binding site for PA1b. Mammalian cells are not sensitive and no binding activity was detected. Signs of apoptosis of the Sf9 cells were observed in response to the toxin. The use of this cellular model also demonstrated that PA1b was internalized in the cells, via the binding site, raising the new question of the role of this toxin within the cell, and of the mechanisms leading to cell death.
|
| 巻・号 |
89(12)
|
| ページ |
1539-43
|
| 公開日 |
2007-12-1
|
| DOI |
10.1016/j.biochi.2007.07.021
|
| PII |
S0300-9084(07)00197-6
|
| PMID |
17845830
|
| MeSH |
Albumins / chemistry*
Animals
Apoptosis / drug effects
Binding Sites
Cell Culture Techniques
Cell Survival / drug effects
Cells, Cultured
Endotoxins / chemistry*
Endotoxins / isolation & purification
Endotoxins / pharmacology
Iodine Radioisotopes / metabolism
Kinetics
Molecular Weight
Peas / metabolism*
Plant Proteins / chemistry
Protein Binding
Protein Isoforms / chemistry
Spodoptera / cytology*
Spodoptera / metabolism*
Spodoptera / ultrastructure
Temperature
Time Factors
|
| IF |
3.413
|
| 引用数 |
12
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
