論文 - 詳細
| RRC ID | 43109 |
|---|---|
| 著者 | Masuda S, Yamamoto K, Hirabayashi T, Ishikawa Y, Ishii T, Kudo I, Murakami M. |
| タイトル | Human group III secreted phospholipase A2 promotes neuronal outgrowth and survival. |
| ジャーナル | Biochem J |
| Abstract |
Human sPLA2-III [group III secreted PLA2 (phospholipase A2)] is an atypical sPLA2 isoenzyme that consists of a central group III sPLA2 domain flanked by unique N- and C-terminal domains. In the present study, we found that sPLA2-III is expressed in neuronal cells, such as peripheral neuronal fibres, spinal DRG (dorsal root ganglia) neurons and cerebellar Purkinje cells. Adenoviral expression of sPLA2-III in PC12 cells (pheochromocytoma cells) or DRG explants facilitated neurite outgrowth, whereas expression of a catalytically inactive sPLA2-III mutant or use of sPLA2-III-directed siRNA (small interfering RNA) reduced NGF (nerve growth factor)-induced neuritogenesis. sPLA2-III also suppressed neuronal death induced by NGF deprivation. Lipid MS revealed that sPLA2-III overexpression increased the cellular level of lysophosphatidylcholine, a PLA2 reaction product with neuritogenic and neurotropic activities, whereas siRNA knockdown reduced the level of lysophosphatidylcholine. These observations suggest the potential contribution of sPLA2-III to neuronal differentiation and its function under certain conditions. |
| 巻・号 | 409(2) |
| ページ | 429-38 |
| 公開日 | 2008-1-15 |
| DOI | 10.1042/BJ20070844 |
| PII | BJ20070844 |
| PMID | 17868035 |
| MeSH | Animals Cell Differentiation Cell Survival / radiation effects DNA, Complementary / metabolism Group III Phospholipases A2 / metabolism* Humans Mice Mice, Inbred C57BL Nerve Growth Factor / metabolism Neurites / physiology Neurons / cytology* Neurons / enzymology* PC12 Cells RNA, Small Interfering / metabolism Rats Spectrometry, Mass, Electrospray Ionization |
| IF | 4.097 |
| 引用数 | 40 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | PC-12(RCB0009) |