RRC ID |
43109
|
著者 |
Masuda S, Yamamoto K, Hirabayashi T, Ishikawa Y, Ishii T, Kudo I, Murakami M.
|
タイトル |
Human group III secreted phospholipase A2 promotes neuronal outgrowth and survival.
|
ジャーナル |
Biochem J
|
Abstract |
Human sPLA2-III [group III secreted PLA2 (phospholipase A2)] is an atypical sPLA2 isoenzyme that consists of a central group III sPLA2 domain flanked by unique N- and C-terminal domains. In the present study, we found that sPLA2-III is expressed in neuronal cells, such as peripheral neuronal fibres, spinal DRG (dorsal root ganglia) neurons and cerebellar Purkinje cells. Adenoviral expression of sPLA2-III in PC12 cells (pheochromocytoma cells) or DRG explants facilitated neurite outgrowth, whereas expression of a catalytically inactive sPLA2-III mutant or use of sPLA2-III-directed siRNA (small interfering RNA) reduced NGF (nerve growth factor)-induced neuritogenesis. sPLA2-III also suppressed neuronal death induced by NGF deprivation. Lipid MS revealed that sPLA2-III overexpression increased the cellular level of lysophosphatidylcholine, a PLA2 reaction product with neuritogenic and neurotropic activities, whereas siRNA knockdown reduced the level of lysophosphatidylcholine. These observations suggest the potential contribution of sPLA2-III to neuronal differentiation and its function under certain conditions.
|
巻・号 |
409(2)
|
ページ |
429-38
|
公開日 |
2008-1-15
|
DOI |
10.1042/BJ20070844
|
PII |
BJ20070844
|
PMID |
17868035
|
MeSH |
Animals
Cell Differentiation
Cell Survival / radiation effects
DNA, Complementary / metabolism
Group III Phospholipases A2 / metabolism*
Humans
Mice
Mice, Inbred C57BL
Nerve Growth Factor / metabolism
Neurites / physiology
Neurons / cytology*
Neurons / enzymology*
PC12 Cells
RNA, Small Interfering / metabolism
Rats
Spectrometry, Mass, Electrospray Ionization
|
IF |
4.097
|
引用数 |
40
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
PC-12(RCB0009) |