論文 - 詳細
| RRC ID | 43117 |
|---|---|
| 著者 | Iwasaki Y, Maie H, Akiyoshi K. |
| タイトル | Cell-specific delivery of polymeric nanoparticles to carbohydrate-tagging cells. |
| ジャーナル | Biomacromolecules |
| Abstract |
Carbohydrates on cell surfaces contribute a variety of communications between the cell and its environment, and they have been assumed to act as markers for cellular recognition. In this research, 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer nanoparticles, which can react with specific carbohydrates of target cells, were newly prepared to serve as novel drug carriers. A water-soluble MPC polymer bearing hydrazide groups (PMBH) was synthesized by conventional radical polymerization. The MPC polymer showed amphiphilic nature and worked as an emulsifier to form nanoparticles. The nanoparticles covered with PMBH were prepared by the solvent evaporation method and exhibited monodispersity. They were approximately 200 nm in diameter and -2.0 mV in surface potential. According to a surface analysis of the nanoparticles, phosphorylcholine and hydrazide groups were observed, and the surface was fully covered with PMBH. Unnatural carbohydrates having ketone groups on human cervical carcinoma cell (HeLa) surfaces were expressed by treatment with levulinoyl mannosamine (ManLev). When the PMBH nanoparticles were in contact with the ManLev-treated HeLa cells, they accumulated in the cells. In contrast, the nanoparticles were not observed in native HeLa cells (without unnatural carbohydrates). These results indicate that the hydrazide groups of the nanoparticles selectively reacted to the ketone groups of the carbohydrates on the cell surface. The PMBH nanoparticles immobilized with anticancer drugs such as doxorubicin or paclitaxel were in contact with either ManLev-treated or untreated HeLa cells. The viability of the ManLev-treated HeLa cells was effectively reduced, but that of the untreated cells was preserved. This indicated that the anticancer drugs were selectively delivered to the ManLev-treated cells. Nonspecific cellular uptake of the nanoparticles was effectively reduced by MPC polymer coating. Furthermore, the immobilization processes of the drugs differed because of the solubility of the drugs. In conclusion, cellular-specific drug delivery by means of the novel nanoparticles was demonstrated with the selective reaction between unnatural carbohydrates on the cell surface and the hydrazide groups bearing the phosphorylcholine polymer nanoparticles. |
| 巻・号 | 8(10) |
| ページ | 3162-8 |
| 公開日 | 2007-10-1 |
| DOI | 10.1021/bm700606z |
| PMID | 17883278 |
| MeSH | Antineoplastic Agents / administration & dosage Carbohydrates / chemistry* Cell Membrane / metabolism* Chemistry, Pharmaceutical / methods* Drug Carriers* Drug Delivery Systems HeLa Cells Humans Methacrylates / chemistry Microscopy, Fluorescence Microscopy, Phase-Contrast Nanoparticles / chemistry* Phosphorylcholine / analogs & derivatives Phosphorylcholine / chemistry Polymers / chemistry Spectrometry, X-Ray Emission |
| IF | 6.092 |
| 引用数 | 39 |
| WOS 分野 | POLYMER SCIENCE CHEMISTRY, ORGANIC BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | |