論文 - 詳細
| RRC ID | 43142 |
|---|---|
| 著者 | Nakano K, Tozuka Y, Takeuchi H. |
| タイトル | Effect of surface properties of liposomes coated with a modified polyvinyl alcohol (PVA-R) on the interaction with macrophage cells. |
| ジャーナル | Int J Pharm |
| Abstract |
The purpose of this study was to investigate the effect of a polymer coating using modified polyvinyl alcohol (PVA-R) on the interaction between liposomes and macrophage cells (J774 cells). The PVA-R-coated liposomes, which were labeled with 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocianin perchlorate (DiI) as a fluorescence reagent, were prepared with the conventional hydration method followed by extrusion and surface modification with PVA-R. The effects of polymer coating on the interaction with J774 cells were evaluated by using flow cytometry and confocal laser scanning microscopy (CLSM). When liposomes with or without PVA-R coating were incubated with J744 cells, the fluorescence emission intensity of DiI from J774 cells was significantly smaller than in the case of non-coated liposomes. This result indicates that decreased interaction of PVA-R-coated liposomes with macrophage cells could be well detected using flow cytometry and CLSM. These in vitro tests explained the differences in blood circulation of polymer-coated liposomes having different lipid formulations in rats. |
| 巻・号 | 354(1-2) |
| ページ | 174-9 |
| 公開日 | 2008-4-16 |
| DOI | 10.1016/j.ijpharm.2007.08.046 |
| PII | S0378-5173(07)00741-7 |
| PMID | 17942254 |
| MeSH | Animals Carbocyanines / metabolism Cells, Cultured Flow Cytometry Fluorescent Dyes / metabolism Hypromellose Derivatives In Vitro Techniques Liposomes Macrophages / drug effects* Macrophages / metabolism Methylcellulose / analogs & derivatives* Methylcellulose / chemistry Mice Microscopy, Confocal Polymers / chemistry* Polyvinyl Alcohol / chemistry* Surface Properties |
| IF | 4.845 |
| 引用数 | 23 |
| WOS 分野 | PHARMACOLOGY & PHARMACY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | |