| RRC ID |
43144
|
| Author |
Kokuryo T, Senga T, Yokoyama Y, Nagino M, Nimura Y, Hamaguchi M.
|
| Title |
Nek2 as an effective target for inhibition of tumorigenic growth and peritoneal dissemination of cholangiocarcinoma.
|
| Journal |
Cancer Res
|
| Abstract |
We investigated the role of Nek2, a member of the serine/threonine kinase family, Nek, in the tumorigenic growth of cholangiocarcinoma cells. Expression of Nek2 is elevated in cholangiocarcinoma in a tumor-specific manner as compared with that of normal fibroblast cells. Expression of exogenous Nek2 did not perturb the growth of cholangiocarcinoma cells, whereas suppression of the Nek2 expression with siRNA resulted in the inhibition of cell proliferation and induced cell death. In xenograft-nude mouse model, s.c. injection of Nek2 siRNA around the tumor nodules resulted in reduction of tumor size as compared with those of control siRNA injection. In peritoneal dissemination model, Nek2 siRNA-treated mice showed statistically longer survival periods in comparison with those of the control siRNA-treated mice. Taken together, our data indicate a pivotal role of Nek2 in tumorigenic growth of cholangiocarcinoma.
|
| Volume |
67(20)
|
| Pages |
9637-42
|
| Published |
2007-10-15
|
| DOI |
10.1158/0008-5472.CAN-07-1489
|
| PII |
67/20/9637
|
| PMID |
17942892
|
| MeSH |
Animals
Bile Duct Neoplasms / enzymology*
Bile Duct Neoplasms / genetics
Bile Duct Neoplasms / pathology
Bile Ducts, Intrahepatic / enzymology*
Bile Ducts, Intrahepatic / pathology
Cell Death / physiology
Cell Growth Processes / physiology
Cell Line, Tumor
Cholangiocarcinoma / enzymology*
Cholangiocarcinoma / genetics
Cholangiocarcinoma / pathology
DNA, Complementary / biosynthesis
DNA, Complementary / genetics
Gene Expression
Humans
Liver / enzymology
Male
Mice
Mice, Nude
NIMA-Related Kinases
Peritoneal Neoplasms / enzymology*
Peritoneal Neoplasms / genetics
Peritoneal Neoplasms / secondary*
Protein Serine-Threonine Kinases / antagonists & inhibitors*
Protein Serine-Threonine Kinases / biosynthesis
Protein Serine-Threonine Kinases / genetics
RNA, Small Interfering / genetics
|
| IF |
9.727
|
| Times Cited |
69
|
|
WOS Category
|
ONCOLOGY
|
| Resource |
| Human and Animal Cells |
HuCCT1
TFK1
HuH28
ETK1
RBE
IHGGK |
