論文 - 詳細
| RRC ID | 43150 |
|---|---|
| 著者 | Takahashi N, Udagawa N, Kobayashi Y, Suda T. |
| タイトル | Generation of osteoclasts in vitro, and assay of osteoclast activity. |
| ジャーナル | Methods Mol Med |
| Abstract |
Osteoclasts are bone-resorbing multinucleated cells derived from the monocyte-macrophage lineage. The authors have developed a mouse marrow culture system and a coculture system of mouse osteoblasts and hemopoietic cells, in which osteoclasts are formed in response to various osteotropic factors such as 1alpha,25-dihydroxyvitamin D3, parathyroid hormone, prostaglandin E2, and interleukin-11. Recent studies have revealed that osteoblasts express two cytokines essential for osteoclastogenesis: receptor activator of nuclear factor kappaB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF). Using RANKL and M-CSF, we can induce osteoclasts from monocyte-macrophage lineage cells even in the absence of osteoblasts. This chapter describes the methods for osteoclast formation in vitro in the presence and absence of osteoblasts, and for pit-formation assay using dentine slices and osteoclasts formed in vitro. These culture systems have made it possible to investigate each step of osteoclast development and function separately. |
| 巻・号 | 135 |
| ページ | 285-301 |
| 公開日 | 2007-1-1 |
| DOI | 10.1007/978-1-59745-401-8_18 |
| PII | 1-59745-401-X:285 |
| PMID | 17951666 |
| MeSH | Actins / metabolism Animals Bone Marrow Cells / cytology Bone Resorption / metabolism Bone Resorption / pathology Buffers Cathepsin K Cathepsins / metabolism Cell Differentiation / drug effects Cell Line Cell Separation / methods Coculture Techniques / methods Collagen Culture Media Gels Immunohistochemistry / methods Macrophage Colony-Stimulating Factor / metabolism Macrophage Colony-Stimulating Factor / pharmacology Macrophages / cytology Mice Models, Biological Osteoblasts / cytology Osteoclasts / cytology* Osteoclasts / drug effects Osteoclasts / metabolism RANK Ligand / metabolism RANK Ligand / pharmacology |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) ST2(RCB0224) MC3T3-G2/PA6(RCB1127) |