Reference - Detail
RRC ID | 43160 |
---|---|
Author | Okamura M, Kobayashi M, Suzuki F, Shimada J, Sakagami H. |
Title | Induction of cell death by combination treatment with cisplatin and 5-fluorouracil in a human oral squamous cell carcinoma cell line. |
Journal | Anticancer Res |
Abstract |
The possible apoptosis-inducing activity of several sequential treatments of cisplatin (CDDP) and 5-fluorouracil (5-FU) against the human oral squamous cell carcinoma HSC-2 cell line was investigated. The following three combination treatments (CT) were used: simultaneous treatment with CDDP and 5-FU (for 72 hours) (CT-1), CDDP treatment (24 hours) followed by 5-FU treatment (48 hours) (CT-2) and 5-FU treatment (24 hours) followed by CDDP treatment (48 hours) (CT-3). CT-1 produced the highest cytotoxicity, followed by CT-3 and CT-2. No treatment induced any detectable internucleosomal DNA fragmentation, and caspase-3,-8 and -9 were activated to a much lesser extent than that attained using actinomycin D. High-performance liquid chromatography analysis demonstrated that 5-FU, as well as CT-1 and CT-2, preferentially reduced the intracellular concentration of putrescine. These results suggest that simultaneous treatment with CDDP and 5-FU induces lower level of apoptotic cell death in HSC-2 cells. |
Volume | 27(5A) |
Pages | 3331-7 |
Published | 2007-1-1 |
PMID | 17970078 |
MeSH | Antineoplastic Combined Chemotherapy Protocols / administration & dosage* Apoptosis / drug effects* Biogenic Polyamines / metabolism Carcinoma, Squamous Cell / drug therapy* Carcinoma, Squamous Cell / metabolism Carcinoma, Squamous Cell / pathology Caspases / metabolism Cell Line, Tumor Cisplatin / administration & dosage DNA Fragmentation Drug Administration Schedule Enzyme Activation Fluorouracil / administration & dosage HL-60 Cells Humans Isoenzymes / metabolism Mouth Neoplasms / drug therapy* Mouth Neoplasms / metabolism Mouth Neoplasms / pathology |
IF | 1.994 |
Times Cited | 13 |
WOS Category | ONCOLOGY |
Resource | |
Human and Animal Cells | HSC-2(RCB1945) |