論文 - 詳細
| RRC ID | 43166 |
|---|---|
| 著者 | Hirasawa A, Itsubo C, Sadakane K, Hara T, Shinagawa S, Koga H, Nose H, Koshimizu TA, Tsujimoto G. |
| タイトル | Production and characterization of a monoclonal antibody against GPR40 (FFAR1; free fatty acid receptor 1). |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
GPR40 is G protein-coupled receptor whose endogenous ligands have recently been identified as free fatty acids (FFAs), and it has been implicated to play an important role in FFA-mediated enhancement of glucose-stimulated insulin release. We have developed a monoclonal antibody against the extracellular domain of GPR40. Specificity of the antibody was demonstrated by immunoprecipitation and cell surface staining using GPR40-transfected cells. GPR40 immunoreactivity was highly abundant in mouse pancreatic beta-cells and splenocytes, THP-1 cells, and human peripheral blood mononuclear cells. The anti-GPR40 monoclonal antibody should prove valuable for further studying the function of this nutrient sensing receptor. |
| 巻・号 | 365(1) |
| ページ | 22-8 |
| 公開日 | 2008-1-4 |
| DOI | 10.1016/j.bbrc.2007.10.142 |
| PII | S0006-291X(07)02281-4 |
| PMID | 17980148 |
| MeSH | Amino Acid Sequence Animals Antibodies, Monoclonal / biosynthesis Antibodies, Monoclonal / chemistry Antibodies, Monoclonal / immunology* Antibody Specificity Cells, Cultured Humans Immunohistochemistry Leukocytes, Mononuclear / metabolism Mice Molecular Sequence Data Peptide Fragments / metabolism Receptors, G-Protein-Coupled / analysis Receptors, G-Protein-Coupled / immunology* Transfection |
| IF | 2.985 |
| 引用数 | 24 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | THP-1(RCB1189) |