RRC ID |
43166
|
著者 |
Hirasawa A, Itsubo C, Sadakane K, Hara T, Shinagawa S, Koga H, Nose H, Koshimizu TA, Tsujimoto G.
|
タイトル |
Production and characterization of a monoclonal antibody against GPR40 (FFAR1; free fatty acid receptor 1).
|
ジャーナル |
Biochem Biophys Res Commun
|
Abstract |
GPR40 is G protein-coupled receptor whose endogenous ligands have recently been identified as free fatty acids (FFAs), and it has been implicated to play an important role in FFA-mediated enhancement of glucose-stimulated insulin release. We have developed a monoclonal antibody against the extracellular domain of GPR40. Specificity of the antibody was demonstrated by immunoprecipitation and cell surface staining using GPR40-transfected cells. GPR40 immunoreactivity was highly abundant in mouse pancreatic beta-cells and splenocytes, THP-1 cells, and human peripheral blood mononuclear cells. The anti-GPR40 monoclonal antibody should prove valuable for further studying the function of this nutrient sensing receptor.
|
巻・号 |
365(1)
|
ページ |
22-8
|
公開日 |
2008-1-4
|
DOI |
10.1016/j.bbrc.2007.10.142
|
PII |
S0006-291X(07)02281-4
|
PMID |
17980148
|
MeSH |
Amino Acid Sequence
Animals
Antibodies, Monoclonal / biosynthesis
Antibodies, Monoclonal / chemistry
Antibodies, Monoclonal / immunology*
Antibody Specificity
Cells, Cultured
Humans
Immunohistochemistry
Leukocytes, Mononuclear / metabolism
Mice
Molecular Sequence Data
Peptide Fragments / metabolism
Receptors, G-Protein-Coupled / analysis
Receptors, G-Protein-Coupled / immunology*
Transfection
|
IF |
2.985
|
引用数 |
24
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
THP-1(RCB1189) |