RRC ID 43180
Author Dutta AK, Korchev YE, Shevchuk AI, Hayashi S, Okada Y, Sabirov RZ.
Title Spatial distribution of maxi-anion channel on cardiomyocytes detected by smart-patch technique.
Journal Biophys J
Abstract Spatial distribution of maxi-anion channels in rat cardiomyocytes were studied by applying the recently developed patch clamp technique under scanning ion conductance microscopy, called the "smart-patch" technique. In primary-cultured neonatal cells, the channel was found to be unevenly distributed over the cell surface with significantly lower channel activity in cellular extensions compared with the other parts. Local ATP release, detected using a PC12 cell-based biosensor technique, also exhibited a similar pattern. The maxi-anion channel activity could not be detected in freshly isolated adult cardiomyocytes by the conventional patch-clamp with 2-MOmega pipettes. However, when fine-tipped 15-20 MOmega pipettes were targeted to only Z-line areas, we observed, for the first time, the maxi-anion events. Smart-patching different regions of the cell surface, we found that the channel activity was maximal at the openings of T-tubules and along Z-lines, but was significantly decreased in the scallop crest area. Thus, it is concluded that maxi-anion channels are concentrated at the openings of T-tubules and along Z-lines in adult cardiomyocytes. This study showed that the smart-patch technique provides a powerful method to detect a unitary event of channels that are localized at some specific site in the narrow region.
Volume 94(5)
Pages 1646-55
Published 2008-3-1
DOI 10.1529/biophysj.107.117820
PII S0006-3495(08)70603-9
PMID 18024498
PMC PMC2242737
MeSH Action Potentials / physiology Adenosine Triphosphate / metabolism Animals Anions Biological Transport Cell Membrane / metabolism* Cell Membrane / ultrastructure Cells, Cultured Ion Channels / analysis* Ion Channels / metabolism* Ion Channels / ultrastructure Microscopy, Electron, Scanning / methods Myocytes, Cardiac / metabolism* Myocytes, Cardiac / ultrastructure PC12 Cells Patch-Clamp Techniques / methods* Rats Rats, Wistar
IF 3.665
Times Cited 37
WOS Category BIOPHYSICS
Resource
Human and Animal Cells