| RRC ID |
43182
|
| 著者 |
Kawano M, Matsuyama K, Miyamae Y, Shinmoto H, Kchouk ME, Morio T, Shigemori H, Isoda H.
|
| タイトル |
Antimelanogenesis effect of Tunisian herb Thymelaea hirsuta extract on B16 murine melanoma cells.
|
| ジャーナル |
Exp Dermatol
|
| Abstract |
Skin pigmentation is the result of melanogenesis that occurs in melanocytes and/or melanoma cells. Although melanogenesis is necessary for the prevention of DNA damage and cancer caused by UV irradiation, excessive accumulation of melanin can also cause melanoma. Thus, we focused on the antimelanogenesis effect of an extract from Thymelaea hirsuta, a Tunisian herb. Murine melanoma B16 cells were treated with T. hirsuta extract, and then cell viability and synthesized melanin content were measured. We found that the T. hirsuta extract decreased the synthesized melanin content in B16 cells without cytotoxicity. Tyrosinase is a key enzyme of melanogenesis and extracellular signal-regulated kinase (ERK)-1/2 phosphorylation is known to be related to melanogenesis inhibition. To clarify its mechanism, we also determined ERK1/2 phosphorylation and tyrosinase expression level. ERK1/2 was immediately phosphorylated in cells just after treatment with the extract. The tyrosinase expression was inhibited after 24 h of stimulation with the extract. The T. hirsuta extract was fractionated, and we found that one fraction considerably decreased the melanin synthesis in B16 cells and that this fraction contains daphnanes as the main component. This indicates that our findings might be attributable to daphnanes.
|
| 巻・号 |
16(12)
|
| ページ |
977-84
|
| 公開日 |
2007-12-1
|
| DOI |
10.1111/j.1600-0625.2007.00618.x
|
| PII |
EXD618
|
| PMID |
18031456
|
| MeSH |
Animals
Cell Line, Tumor
Down-Regulation
MAP Kinase Signaling System / physiology
Melanins / biosynthesis*
Melanoma, Experimental / drug therapy*
Mice
Monophenol Monooxygenase / metabolism
Plant Extracts / chemistry
Plant Extracts / therapeutic use*
Thymelaeaceae / chemistry*
|
| IF |
3.368
|
| 引用数 |
41
|
|
WOS 分野
|
DERMATOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
