RRC ID 43185
Author Kobayashi K, Tada K, Itabe H, Ueno T, Liu PH, Tsutsumi A, Kuwana M, Yasuda T, Shoenfeld Y, de Groot PG, Matsuura E.
Title Distinguished effects of antiphospholipid antibodies and anti-oxidized LDL antibodies on oxidized LDL uptake by macrophages.
Journal Lupus
Abstract Several interpretations have been made regarding the specificity of antiphospholipid antibodies and antibodies against oxidized low-density lipoprotein (oxLDL), but these are still controversial. In the present study, we delineated specificity of these two types of antibodies and analyzed their regulatory effect on oxLDL and/or beta( 2)-glycoprotein I (beta(2)GPI) binding to macrophages. Scavenger receptor-mediated binding of oxLDL (or its beta(2)GPI complexes) to macrophages was observed and the binding was partly prevented by beta( 2)GPI. The IgG monoclonal anti-beta(2)GPI antibody (WB-CAL-1), which was derived from NZW x BXSB F1 mouse (a model of antiphospholipid syndrome), significantly increased the oxLDL/beta(2)GPI binding to macrophages. In contrast, IgM anti-oxLDL natural antibody, EO6 (derived from apoe( -/-) mouse), prevented the binding. Different antigenic specificity of these antibodies to oxLDL and its beta(2)GPI complexes was also confirmed in TLC-ligand blot and ELISA. Thus, IgG anti-beta(2) GPI autoantibodies contribute to lipid metabolism (housekeeping of oxLDL by macrophages) whereas IgM natural anti-oxLDL antibodies may protect against atherogenesis. In addition, in vitro data suggest that relatively high dose of intravenous immunoglobulin preparations (mainly contain IgG anti-oxLDL antibodies) might also prevent atherogenesis by inhibiting the oxLDL binding to macrophages.
Volume 16(12)
Pages 929-38
Published 2007
DOI 10.1177/0961203307084170
PII 16/12/929
PMID 18042586
MeSH Animals Antibodies, Antiphospholipid / physiology* Antibody Specificity Antiphospholipid Syndrome / immunology* Atherosclerosis / immunology* Cell Line Humans Immunoglobulin G / immunology Immunoglobulin G / physiology Immunoglobulin M / immunology Immunoglobulin M / physiology Immunoglobulins, Intravenous / immunology Lipoproteins, LDL / immunology* Lipoproteins, LDL / metabolism Macrophages / immunology Macrophages / physiology* Mice beta 2-Glycoprotein I / physiology*
IF 2.924
Times Cited 22
Human and Animal Cells