論文 - 詳細
| RRC ID | 43197 |
|---|---|
| 著者 | Miyawaki J, Kamei S, Sakayama K, Yamamoto H, Masuno H. |
| タイトル | 4-tert-octylphenol regulates the differentiation of C3H10T1/2 cells into osteoblast and adipocyte lineages. |
| ジャーナル | Toxicol Sci |
| Abstract |
The aim of this study was to investigate whether 4-tert-octylphenol (OP) affects the differentiation of multipotent C3H10T1/2 cells, a cell line established from mouse embryonic connective tissue, into osteoblast and adipocyte lineages. Confluent C3H10T1/2 cells were incubated for 7 days with (OP-treated cultures) or without (control cultures) 15 microg/ml of OP. The 7-day treatment of confluent cells with OP decreased alkaline phosphatase activity by 81%, inhibited the expression of transforming growth factor beta2, and inhibited the morphological changes in cells to an osteoblastic appearance. These results indicate that the 7-day treatment of confluent C3H10T1/2 cells with OP inhibited their differentiation into osteoblasts. Since this treatment strongly induced the expression of peroxisome proliferator-activated receptor r (PPARr) but did not stimulate triacylglycerol (TG) accumulation in cells, C3H10T1/2 cells in the control and OP-treated cultures were incubated for 2 days with a hormone mixture (insulin [INS], dexamethasone, and 1-methyl-3-isobutylxanthine) and incubated for an additional 5 days with INS alone. The TG and adiponectin contents of the OP-treated cultures were 4.2 and 4.1 times higher, respectively, than those of the control cultures. There were many more Oil Red O-staining cells in the OP-treated cultures than in the control cultures. The expression of PPARr in the OP-treated cultures was higher than that in the control cultures. These results indicate that the OP-treated cultures contained a larger number of adipocytes than the control cultures. In conclusion, treatment of C3H10T1/2 cells with OP inhibited osteoblast differentiation, causing a lineage shift toward adipocytes. |
| 巻・号 | 102(1) |
| ページ | 82-8 |
| 公開日 | 2008-3-1 |
| DOI | 10.1093/toxsci/kfm296 |
| PII | kfm296 |
| PMID | 18065773 |
| MeSH | Adipocytes / drug effects* Adipocytes / metabolism Adiponectin / metabolism Alkaline Phosphatase / metabolism Animals Benzhydryl Compounds Cell Differentiation / drug effects* Cell Line Cell Lineage* Cell Shape / drug effects Dose-Response Relationship, Drug Embryonic Stem Cells / drug effects* Embryonic Stem Cells / enzymology Embryonic Stem Cells / metabolism Epoxy Compounds / pharmacology Mice Mice, Inbred C3H Multipotent Stem Cells / drug effects* Multipotent Stem Cells / enzymology Multipotent Stem Cells / metabolism Osteoblasts / drug effects* Osteoblasts / enzymology Osteoblasts / metabolism Peroxisome Proliferator-Activated Receptors / antagonists & inhibitors Peroxisome Proliferator-Activated Receptors / metabolism Phenols / toxicity* Time Factors Transforming Growth Factor beta2 / metabolism Triglycerides / metabolism Water Pollutants, Chemical / toxicity* |
| IF | 3.703 |
| 引用数 | 16 |
| WOS 分野 | TOXICOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 10T1/2(RCB0247) |