論文 - 詳細
| RRC ID | 43208 |
|---|---|
| 著者 | Khodadadi I, Griffin B, Thumser A. |
| タイトル | Differential effects of long-chain fatty acids and clofibrate on gene expression profiles in cardiomyocytes. |
| ジャーナル | Arch Iran Med |
| Abstract |
BACKGROUND:The link between dietary fat and coronary heart disease has attracted much attention since the effect of long-chain fatty acids on gene transcription has been established. The aim of this study was to investigate the effects of long-chain fatty acids and clofibrate on mRNA levels of specific lipid metabolism-related genes and to determine their effects on global transcriptome levels in a cardiovascular cell-line. METHODS:After culturing P19CL6 cells with long-chain fatty acids or clofibrate, the expression levels of heart-type fatty-acid binding protein and peroxisome proliferators-activated receptors (PPARalpha,beta,gamma) were determined by reverse transcriptase-polymerase chain reaction. Additionally, global transcriptome profiles were compared using microarray analysis. RESULTS:Long-chain fatty acids significantly increased the abundance of PPAR and PPAR. Moreover, microarray analysis showed that the effects of linoleic and -linolenic acids, and clofibrate were similar but differed from those of palmitic and oleic acids. CONCLUSION:These findings show that cellular responses to polyunsaturated fatty acids differ from those observed with saturated and monounsaturated fatty acids. |
| 巻・号 | 11(1) |
| ページ | 42-9 |
| 公開日 | 2008-1-1 |
| PII | 0011 |
| PMID | 18154422 |
| MeSH | Analysis of Variance Cells, Cultured Clofibrate / pharmacology* Fatty Acids / pharmacology* Gene Expression Regulation / drug effects* Myocytes, Cardiac / metabolism* Reverse Transcriptase Polymerase Chain Reaction Transcription Factors / metabolism |
| IF | 0.996 |
| 引用数 | 5 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | |