| RRC ID |
43210
|
| 著者 |
Yeh CM, Yeh CK, Hsu XY, Luo QM, Lin MY.
|
| タイトル |
Extracellular expression of a functional recombinant Ganoderma lucidium immunomodulatory protein by Bacillus subtilis and Lactococcus lactis.
|
| ジャーナル |
Appl Environ Microbiol
|
| Abstract |
Bacillus subtilis and Lactococcus lactis are ideal hosts for the production of extracellular heterologous proteins of major commercial importance. A recombinant gene for the novel Ganoderma lucidium immunomodulatory protein LZ-8, recombinant LZ-8, was designed encoding the same amino acid sequence but using the preferred codons for both strains and was synthesized by overlapping extension PCR. Using the signal peptide (SP) from subtilisin YaB (SP(YaB)), recombinant LZ-8 was expressed extracellularly in Bacillus subtilis and Lactococcus lactis. In the absence of SP(YaB), recombinant LZ-8 was expressed extracellularly in B. subtilis, but not in L. lactis. The three expressed recombinant LZ-8s showed different capacities for modulating the production of Th1 and Th2 cytokines by peripheral blood mononuclear cells and of tumor necrosis factor alpha by a macrophage cell line.
|
| 巻・号 |
74(4)
|
| ページ |
1039-49
|
| 公開日 |
2008-2-1
|
| DOI |
10.1128/AEM.01547-07
|
| PII |
AEM.01547-07
|
| PMID |
18156317
|
| PMC |
PMC2258568
|
| MeSH |
Animals
Bacillus subtilis / metabolism
Blotting, Western
Cell Line
Cell Proliferation
Circular Dichroism
Culture Media
Cytokines / metabolism
Electrophoresis, Polyacrylamide Gel
Fungal Proteins / genetics
Fungal Proteins / metabolism*
Humans
Lactococcus lactis / metabolism
Mice
Protein Conformation
Protein Engineering / methods*
Recombinant Proteins / genetics
Recombinant Proteins / metabolism*
Reishi / genetics*
Sequence Analysis, Protein
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
|
| IF |
4.016
|
| 引用数 |
29
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
RAW 264(RCB0535) |
