論文 - 詳細
| RRC ID | 43210 |
|---|---|
| 著者 | Yeh CM, Yeh CK, Hsu XY, Luo QM, Lin MY. |
| タイトル | Extracellular expression of a functional recombinant Ganoderma lucidium immunomodulatory protein by Bacillus subtilis and Lactococcus lactis. |
| ジャーナル | Appl Environ Microbiol |
| Abstract |
Bacillus subtilis and Lactococcus lactis are ideal hosts for the production of extracellular heterologous proteins of major commercial importance. A recombinant gene for the novel Ganoderma lucidium immunomodulatory protein LZ-8, recombinant LZ-8, was designed encoding the same amino acid sequence but using the preferred codons for both strains and was synthesized by overlapping extension PCR. Using the signal peptide (SP) from subtilisin YaB (SP(YaB)), recombinant LZ-8 was expressed extracellularly in Bacillus subtilis and Lactococcus lactis. In the absence of SP(YaB), recombinant LZ-8 was expressed extracellularly in B. subtilis, but not in L. lactis. The three expressed recombinant LZ-8s showed different capacities for modulating the production of Th1 and Th2 cytokines by peripheral blood mononuclear cells and of tumor necrosis factor alpha by a macrophage cell line. |
| 巻・号 | 74(4) |
| ページ | 1039-49 |
| 公開日 | 2008-2-1 |
| DOI | 10.1128/AEM.01547-07 |
| PII | AEM.01547-07 |
| PMID | 18156317 |
| PMC | PMC2258568 |
| MeSH | Animals Bacillus subtilis / metabolism Blotting, Western Cell Line Cell Proliferation Circular Dichroism Culture Media Cytokines / metabolism Electrophoresis, Polyacrylamide Gel Fungal Proteins / genetics Fungal Proteins / metabolism* Humans Lactococcus lactis / metabolism Mice Protein Conformation Protein Engineering / methods* Recombinant Proteins / genetics Recombinant Proteins / metabolism* Reishi / genetics* Sequence Analysis, Protein Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| IF | 4.016 |
| 引用数 | 29 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |