RRC ID 4326
Author Darland-Ransom M, Wang X, Sun CL, Mapes J, Gengyo-Ando K, Mitani S, Xue D.
Title Role of C. elegans TAT-1 protein in maintaining plasma membrane phosphatidylserine asymmetry.
Journal Science
Abstract The asymmetrical distribution of phospholipids on the plasma membrane is critical for maintaining cell integrity and physiology and for regulating intracellular signaling and important cellular events such as clearance of apoptotic cells. How phospholipid asymmetry is established and maintained is not fully understood. We report that the Caenorhabditis elegans P-type adenosine triphosphatase homolog, TAT-1, is critical for maintaining cell surface asymmetry of phosphatidylserine (PS). In animals deficient in tat-1, PS is abnormally exposed on the cell surface, and normally living cells are randomly lost through a mechanism dependent on PSR-1, a PS-recognizing phagocyte receptor, and CED-1, which contributes to recognition and engulfment of apoptotic cells. Thus, tat-1 appears to function in preventing appearance of PS in the outer leaflet of plasma membrane, and ectopic exposure of PS on the cell surface may result in removal of living cells by neighboring phagocytes.
Volume 320(5875)
Pages 528-31
Published 2008-4-25
DOI 10.1126/science.1155847
PII 320/5875/528
PMID 18436785
MeSH Animals Animals, Genetically Modified Apoptosis Caenorhabditis elegans / cytology Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Membrane / metabolism* Germ Cells / cytology Germ Cells / metabolism Muscle Cells / cytology Muscle Cells / metabolism Neurons / cytology Neurons / metabolism Phagocytosis Phosphatidylserines / metabolism* Phospholipid Transfer Proteins / genetics Phospholipid Transfer Proteins / metabolism* RNA Interference Recombinant Fusion Proteins / metabolism
IF 41.846
Times Cited 96
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
C.elegans tm1034 tm1275 tm469