| RRC ID |
4328
|
| 著者 |
Hisamoto N, Moriguchi T, Urushiyama S, Mitani S, Shibuya H, Matsumoto K.
|
| タイトル |
Caenorhabditis elegans WNK-STE20 pathway regulates tube formation by modulating ClC channel activity.
|
| ジャーナル |
EMBO Rep
|
| Abstract |
WNK kinases are a small group of unique serine/threonine protein kinases that are conserved among multicellular organisms. Mutations in WNK1-4 cause pseudohypoaldosteronism type II-a form of hypertension. WNKs have been linked to the STE20 kinases and ion carriers, but the underlying molecular mechanisms by which WNKs regulate cellular processes in whole animals are unknown. The Caenorhabditis elegans WNK-like kinase WNK-1 interacts with and phosphorylates germinal centre kinase (GCK)-3--a STE20-like kinase--which is known to inactivate CLH-3, a CIC chloride channel. The wnk-1 or gck-3 deletion mutation causes an Exc phenotype, a defect in the tubular extension of excretory canals. Expression of the activated form of GCK-3 or the clh-3 deletion mutation can partly suppress wnk-1 or gck-3 defects, respectively. These results indicate that WNK-1 controls the tubular formation of excretory canals by activating GCK-3, resulting in downregulation of CIC channel activity.
|
| 巻・号 |
9(1)
|
| ページ |
70-5
|
| 公開日 |
2008-1-1
|
| DOI |
10.1038/sj.embor.7401128
|
| PII |
7401128
|
| PMID |
18049475
|
| PMC |
PMC2246628
|
| MeSH |
Animals
Caenorhabditis elegans / anatomy & histology*
Caenorhabditis elegans / cytology
Caenorhabditis elegans / enzymology*
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Cell Line
Chloride Channels / metabolism*
Humans
Models, Biological
Mutation / genetics
Protein Serine-Threonine Kinases / genetics
Protein Serine-Threonine Kinases / metabolism*
WNK Lysine-Deficient Protein Kinase 1
|
| IF |
7.497
|
| 引用数 |
27
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| 線虫 |
tm487
tm1296
tm1926 |
