| RRC ID |
43294
|
| Author |
Hiratsuka K, Yin SA, Ohtomo T, Fujita M, Ohtsuki K, Isaka H, Suga T, Kurosawa T, Yamada J.
|
| Title |
Intratesticular localization of the organic solute carrier protein, OSCP1, in spermatogenic cells in mice.
|
| Journal |
Mol Reprod Dev
|
| Abstract |
Organic solute carrier protein 1 (OSCP1) is a recently described human gene that facilitates the transport of various organic solutes into the cell, when expressed in frog eggs. In this study, we cloned a mouse ortholog of OSCP1 encoding 379 amino acid protein, with 94% homology to the human counterpart. The mouse OSCP1 mRNA was predominantly expressed in the testis, in which it was attributed to the spermatogenic cells, except the spermatogonia. Immunohistochemistry confirmed that OSCP1 protein is continuously expressed during spermatogenesis in a stage- and cell type-specific manner, in the leptotene spermatocytes at stage IX through step 15 spermatids. Subcellular fractionation of mouse testis homogenates indicated that OSCP1 is a 45-kDa cytosolic protein. Moreover, when green fluorescent protein-OSCP1 fusion constructs were transfected into cultured cells, the fluorescence localized evenly in the cytoplasm. These results suggest that mouse testis OSCP1 may indirectly mediate substrate uptake into meiotic and spermiogenic germ cells, within the cytosol.
|
| Volume |
75(10)
|
| Pages |
1495-504
|
| Published |
2008-10-1
|
| DOI |
10.1002/mrd.20893
|
| PMID |
18324622
|
| MeSH |
Animals
Cytosol / metabolism
Male
Membrane Transport Proteins / metabolism*
Mice
Mice, Inbred ICR
Spermatogenesis
Spermatogonia / metabolism*
Testis / metabolism*
|
| IF |
2.823
|
| Times Cited |
12
|
|
WOS Category
|
REPRODUCTIVE BIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
DEVELOPMENTAL BIOLOGY
CELL BIOLOGY
|
| Resource |
| Human and Animal Cells |
|
