Reference - Detail
| RRC ID | 43303 |
|---|---|
| Author | Yeasmin S, Nakayama K, Ishibashi M, Katagiri A, Iida K, Purwana IN, Nakayama N, Miyazaki K. |
| Title | Expression of the bric-a-brac tramtrack broad complex protein NAC-1 in cervical carcinomas seems to correlate with poorer prognosis. |
| Journal | Clin Cancer Res |
| Abstract |
PURPOSE:Recent studies have suggested a novel oncogenic role of a bric-a-brac tramtrack broad complex (also known as POZ) domain gene, NAC-1, in ovarian carcinomas. The aim of this study was to clarify the functional role of NAC-1 in human cervical carcinomas. EXPERIMENTAL DESIGN:NAC-1 expression in cervical cancer was assessed by immunohistochemistry, and data on clinical variables were collected by retrospective chart review. NAC-1 gene knockdown using small interfering RNA and a NAC-1 gene transfection system were used to asses NAC-1 function in cervical cancer in vivo. RESULTS:Immunohistochemical and gene expression analysis revealed that NAC-1 is significantly overexpressed in cervical adenocarcinomas and adenosquamous carcinomas compared with squamous cell carcinomas. Patients with squamous cell carcinomas positive for NAC-1 expression who received radiotherapy had significantly shorter overall survival than peers whose tumors did not express NAC-1, and multivariate analysis showed that NAC-1 expression was an independent prognostic factor for overall survival after radiotherapy. Overexpressions of the NAC-1 gene stimulated cell proliferation in cervical carcinoma cells of the TCS, CaSki, and HeLa P3 lines, which do not have endogenous NAC-1 expression. NAC-1 gene knockdown inhibited cell growth and induced apoptosis in HeLa, HeLa TG, and ME180 cells, all of which overexpressed NAC-1. CONCLUSIONS:Our findings suggest that NAC-1 may play an important role in cervical carcinomas; moreover, these findings provide a rationale for future development of NAC-1-based therapy for cervical carcinomas that overexpress this candidate oncogene. |
| Volume | 14(6) |
| Pages | 1686-91 |
| Published | 2008-3-15 |
| DOI | 10.1158/1078-0432.CCR-07-4085 |
| PII | 14/6/1686 |
| PMID | 18347169 |
| MeSH | Adenocarcinoma / diagnosis* Adenocarcinoma / genetics Adenocarcinoma / mortality Adenocarcinoma / radiotherapy Adult Aged Aged, 80 and over Apoptosis / drug effects Carcinoma, Squamous Cell / diagnosis Carcinoma, Squamous Cell / genetics Carcinoma, Squamous Cell / mortality Carcinoma, Squamous Cell / radiotherapy Female Gene Expression Regulation, Neoplastic HeLa Cells Humans Middle Aged Multiprotein Complexes / genetics Multiprotein Complexes / metabolism Neoplasm Proteins / antagonists & inhibitors Neoplasm Proteins / genetics Neoplasm Proteins / metabolism* Prognosis RNA, Small Interfering / pharmacology Repressor Proteins / antagonists & inhibitors Repressor Proteins / genetics Repressor Proteins / metabolism* Survival Analysis Tumor Cells, Cultured Uterine Cervical Neoplasms / diagnosis* Uterine Cervical Neoplasms / genetics Uterine Cervical Neoplasms / mortality Uterine Cervical Neoplasms / radiotherapy |
| IF | 10.107 |
| Times Cited | 33 |
| WOS Category | ONCOLOGY |
| Resource | |
| Human and Animal Cells | TCS(RCB0638) |