| RRC ID |
43337
|
| Author |
Kouro T, Yokota T, Welner R, Kincade PW.
|
| Title |
In vitro differentiation and measurement of B cell progenitor activity in culture.
|
| Journal |
Curr Protoc Immunol
|
| Abstract |
As well as other blood cells, B lymphocytes originate from hematopoietic stem cells. However, it is not fully understood how their production is controlled. In the serum-free, stromal-cell-free B cell differentiation culture described here, early steps of the B lineage differentiation process are reproduced under defined conditions. This assay is useful for examining the direct effects of various soluble factors on B cell progenitors because it does not contain stromal cells or unknown factors. Additionally, this assay yields sufficient cloning to measure B cell progenitors from single cell cultures. Stromal cell coculture assays are also described that cover a wider category of precursors such as human B cell progenitors.
|
| Volume |
Chapter 22
|
| Pages |
Unit 22F.2
|
| Published |
2005-5-1
|
| DOI |
10.1002/0471142735.im22f02s66
|
| PMID |
18432949
|
| MeSH |
Animals
B-Lymphocytes / cytology*
Cell Differentiation
Cells, Cultured
Clinical Laboratory Techniques*
Mice
Stem Cells / cytology*
|
| Resource |
| Human and Animal Cells |
|
