Reference - Detail
| RRC ID | 4338 |
|---|---|
| Author | Duchaine TF, Wohlschlegel JA, Kennedy S, Bei Y, Conte D Jr, Pang K, Brownell DR, Harding S, Mitani S, Ruvkun G, Yates JR 3rd, Mello CC. |
| Title | Functional proteomics reveals the biochemical niche of C. elegans DCR-1 in multiple small-RNA-mediated pathways. |
| Journal | Cell |
| Abstract |
In plants, animals, and fungi, members of the Dicer family of RNase III-related enzymes process double-stranded RNA (dsRNA) to initiate small-RNA-mediated gene-silencing mechanisms. To learn how C. elegans Dicer, DCR-1, functions in multiple distinct silencing mechanisms, we used a mass-spectrometry-based proteomics approach to identify DCR-1-interacting proteins. We then generated and characterized deletion alleles for the corresponding genes. The interactors are required for production of three species of small RNA, including (1) small interfering RNAs (siRNAs), derived from exogenous dsRNA triggers (exo-siRNAs); (2) siRNAs derived from endogenous triggers (endo-siRNAs); and (3) developmental regulatory microRNAs (miRNAs). One interactor, the conserved RNA-phosphatase homolog PIR-1, is required for the processing of a putative amplified DCR-1 substrate. Interactors required for endo-siRNA production include ERI-1 and RRF-3, whose loss of function enhances RNAi. Our findings provide a first glimpse at the complex biochemical niche of Dicer and suggest that competition exists between DCR-1-mediated small-RNA pathways. |
| Volume | 124(2) |
| Pages | 343-54 |
| Published | 2006-1-27 |
| DOI | 10.1016/j.cell.2005.11.036 |
| PII | S0092-8674(05)01394-2 |
| PMID | 16439208 |
| MeSH | Amino Acid Sequence Animals Binding, Competitive Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism DNA-Binding Proteins / chemistry DNA-Binding Proteins / genetics* DNA-Binding Proteins / metabolism Endoribonucleases / genetics* Endoribonucleases / metabolism Exoribonucleases / genetics Exoribonucleases / metabolism Gene Deletion Mass Spectrometry MicroRNAs / metabolism* Models, Biological Molecular Sequence Data Molecular Structure Proteomics RNA Interference RNA, Small Interfering / metabolism* RNA-Dependent RNA Polymerase / genetics RNA-Dependent RNA Polymerase / metabolism Ribonuclease III Sequence Alignment Signal Transduction / genetics* |
| IF | 38.637 |
| Times Cited | 267 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | F1000Research |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm1496 tm1217 tm1361 tm1705 |