| RRC ID |
43395
|
| 著者 |
Kyogoku K, Yoshida K, Watanabe H, Yamashita T, Kawabe Y, Motono M, Nishijima K, Kamihira M, Iijima S.
|
| タイトル |
Production of recombinant tumor necrosis factor receptor/Fc fusion protein by genetically manipulated chickens.
|
| ジャーナル |
J Biosci Bioeng
|
| Abstract |
We previously reported the production of recombinant proteins using genetically manipulated chickens and quails. In this study, we constructed a retroviral vector encoding an expression cassette for a fusion protein of the extracellular domain of the human tumor necrosis factor (TNF) receptor 2 and Fc region of human IgG1 (TNFR/Fc), which is expected as an effective drug for inflammatory diseases such as rheumatoid arthritis. The concentrated viral vector was injected into developing chicken embryos. The chickens that hatched stably produced TNFR/Fc in the serum and egg yolk for six months. It appears that the fused protein is transported and accumulated into yolk from the serum, which is mediated by the Fc receptor. The protein purified from the yolk and serum inhibited the cytotoxic activity of TNF-* toward L929 cells, indicating that the protein produced by the chickens is biologically active. These results indicate the effectiveness of the recovery of Fc-fused proteins from the yolk of genetically manipulated chickens.
|
| 巻・号 |
105(5)
|
| ページ |
454-9
|
| 公開日 |
2008-5-1
|
| DOI |
10.1263/jbb.105.454
|
| PII |
S1389-1723(08)70094-X
|
| PMID |
18558334
|
| MeSH |
Animals
Animals, Genetically Modified / physiology*
Chickens / genetics*
Chickens / metabolism*
Egg Yolk / metabolism*
Immunoglobulin G / genetics
Immunoglobulin G / metabolism*
Protein Engineering / methods*
Recombinant Fusion Proteins / metabolism*
Tumor Necrosis Factor-alpha / genetics
Tumor Necrosis Factor-alpha / metabolism*
|
| IF |
2.366
|
| 引用数 |
20
|
|
WOS 分野
|
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
L929(RCB0535) |
