論文 - 詳細
| RRC ID | 43421 |
|---|---|
| 著者 | Kommineni VK, Nagineni CN, William A, Detrick B, Hooks JJ. |
| タイトル | IFN-gamma acts as anti-angiogenic cytokine in the human cornea by regulating the expression of VEGF-A and sVEGF-R1. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Inflammatory processes within the cornea are known to be associated with corneal neovascularization (CN). We examined the effects of inflammatory mediators on the expression of angiogenic factors by corneal cells. TNF-alpha and IL-1 induced VEGF-A secretion by corneal fibroblasts (HCRF) and this was inhibited significantly by IFN-gamma. Constitutively secreted VEGF-A by corneal epithelial cells (HCE) was not affected by these cytokines. Moreover, sVEGF-R1(sFlt-1) secretion by HCRF was stimulated significantly by IFN-gamma. JAK-STAT pathway inhibitor reversed the effects of IFN-gamma on VEGF-A and sFlt-1 secretion by HCRF. RT-PCR analysis showed that IFN-gamma influences the expression of VEGF-A and sFlt-1 by affecting their mRNA level. IFN-gamma inhibited TGF-beta induced VEGF-A secretion but not sVEGF-R1 secretion. This is the first report demonstrating the inhibitory and stimulatory effects of IFN-gamma on VEGF-A and sFlt-1 secretion, respectively. Our results suggest that IFN-gamma acts as an anti-angiogenic cytokine in the human cornea. |
| 巻・号 | 374(3) |
| ページ | 479-84 |
| 公開日 | 2008-9-26 |
| DOI | 10.1016/j.bbrc.2008.07.042 |
| PII | S0006-291X(08)01367-3 |
| PMID | 18639520 |
| PMC | PMC2997485 |
| MeSH | Cell Line Cornea / drug effects Cornea / enzymology* Corneal Neovascularization / enzymology* Fibroblasts / drug effects Fibroblasts / enzymology Humans Interferon-gamma / pharmacology Interferon-gamma / physiology* Interleukin-1 / pharmacology Janus Kinase 1 / antagonists & inhibitors RNA, Messenger / biosynthesis Receptor, Fibroblast Growth Factor, Type 1 / agonists* Receptor, Fibroblast Growth Factor, Type 1 / metabolism Transforming Growth Factor beta / pharmacology Vascular Endothelial Growth Factor A / antagonists & inhibitors* Vascular Endothelial Growth Factor A / metabolism |
| IF | 2.985 |
| 引用数 | 26 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | |