| RRC ID |
43421
|
| 著者 |
Kommineni VK, Nagineni CN, William A, Detrick B, Hooks JJ.
|
| タイトル |
IFN-gamma acts as anti-angiogenic cytokine in the human cornea by regulating the expression of VEGF-A and sVEGF-R1.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Inflammatory processes within the cornea are known to be associated with corneal neovascularization (CN). We examined the effects of inflammatory mediators on the expression of angiogenic factors by corneal cells. TNF-alpha and IL-1 induced VEGF-A secretion by corneal fibroblasts (HCRF) and this was inhibited significantly by IFN-gamma. Constitutively secreted VEGF-A by corneal epithelial cells (HCE) was not affected by these cytokines. Moreover, sVEGF-R1(sFlt-1) secretion by HCRF was stimulated significantly by IFN-gamma. JAK-STAT pathway inhibitor reversed the effects of IFN-gamma on VEGF-A and sFlt-1 secretion by HCRF. RT-PCR analysis showed that IFN-gamma influences the expression of VEGF-A and sFlt-1 by affecting their mRNA level. IFN-gamma inhibited TGF-beta induced VEGF-A secretion but not sVEGF-R1 secretion. This is the first report demonstrating the inhibitory and stimulatory effects of IFN-gamma on VEGF-A and sFlt-1 secretion, respectively. Our results suggest that IFN-gamma acts as an anti-angiogenic cytokine in the human cornea.
|
| 巻・号 |
374(3)
|
| ページ |
479-84
|
| 公開日 |
2008-9-26
|
| DOI |
10.1016/j.bbrc.2008.07.042
|
| PII |
S0006-291X(08)01367-3
|
| PMID |
18639520
|
| PMC |
PMC2997485
|
| MeSH |
Cell Line
Cornea / drug effects
Cornea / enzymology*
Corneal Neovascularization / enzymology*
Fibroblasts / drug effects
Fibroblasts / enzymology
Humans
Interferon-gamma / pharmacology
Interferon-gamma / physiology*
Interleukin-1 / pharmacology
Janus Kinase 1 / antagonists & inhibitors
RNA, Messenger / biosynthesis
Receptor, Fibroblast Growth Factor, Type 1 / agonists*
Receptor, Fibroblast Growth Factor, Type 1 / metabolism
Transforming Growth Factor beta / pharmacology
Vascular Endothelial Growth Factor A / antagonists & inhibitors*
Vascular Endothelial Growth Factor A / metabolism
|
| IF |
2.985
|
| 引用数 |
26
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
