RRC ID 43439
著者 Shida D, Fang X, Kordula T, Takabe K, Lépine S, Alvarez SE, Milstien S, Spiegel S.
タイトル Cross-talk between LPA1 and epidermal growth factor receptors mediates up-regulation of sphingosine kinase 1 to promote gastric cancer cell motility and invasion.
ジャーナル Cancer Res
Abstract Lysophosphatidic acid (LPA) and sphingosine-1-phosphate (S1P) are lysophospholipid mediators of diverse cellular processes important for cancer progression. S1P is produced by two sphingosine kinases, SphK1 and SphK2. Expression of SphK1 is elevated in many cancers. Here, we report that LPA markedly enhanced SphK1 mRNA and protein in gastric cancer MKN1 cells but had no effect on SphK2. LPA also up-regulated SphK1 expression in other human cancer cells that endogenously express the LPA(1) receptor, such as DLD1 colon cancer cells and MDA-MB-231 breast cancer cells, but not in HT29 colon cancer cells or MDA-MB-453 breast cancer cells, which do not express the LPA(1) receptor. An LPA(1) receptor antagonist or down-regulation of its expression prevented SphK1 and S1P(3) receptor up-regulation by LPA. LPA transactivated the epidermal growth factor receptor (EGFR) in these cells, and the EGFR inhibitor AG1478 attenuated the increased SphK1 and S1P(3) expression induced by LPA. Moreover, down-regulation of SphK1 attenuated LPA-stimulated migration and invasion of MNK1 cells yet had no effect on expression of neovascularizing factors, such as interleukin (IL)-8, IL-6, urokinase-type plasminogen activator (uPA), or uPA receptor induced by LPA. Finally, down-regulation of S1P(3), but not S1P(1), also reduced LPA-stimulated migration and invasion of MKN1 cells. Collectively, our results suggest that SphK1 is a convergence point of multiple cell surface receptors for three different ligands, LPA, EGF, and S1P, which have all been implicated in regulation of motility and invasiveness of cancer cells.
巻・号 68(16)
ページ 6569-77
公開日 2008-8-15
DOI 10.1158/0008-5472.CAN-08-0411
PII 68/16/6569
PMID 18701480
PMC PMC2763580
MeSH Blotting, Western Breast Neoplasms / genetics Breast Neoplasms / metabolism Breast Neoplasms / pathology Cell Movement / physiology* Cell Proliferation Chemotaxis Colonic Neoplasms / genetics Colonic Neoplasms / metabolism Colonic Neoplasms / pathology ErbB Receptors / metabolism* Humans Interleukin-6 / genetics Interleukin-6 / metabolism Interleukin-8 / genetics Interleukin-8 / metabolism Lysophospholipids / metabolism* Neoplasm Invasiveness Phosphotransferases (Alcohol Group Acceptor) / genetics Phosphotransferases (Alcohol Group Acceptor) / metabolism* RNA, Messenger Receptors, Lysophosphatidic Acid / metabolism* Receptors, Lysosphingolipid / genetics Receptors, Lysosphingolipid / metabolism Reverse Transcriptase Polymerase Chain Reaction Sphingosine / analogs & derivatives Sphingosine / metabolism Stomach Neoplasms / genetics Stomach Neoplasms / metabolism Stomach Neoplasms / pathology* Transcriptional Activation Tumor Cells, Cultured Up-Regulation Urokinase-Type Plasminogen Activator / genetics Urokinase-Type Plasminogen Activator / metabolism
IF 9.727
引用数 90
WOS 分野 ONCOLOGY
リソース情報
ヒト・動物細胞 MKN1(RCB1003)