論文 - 詳細
| RRC ID | 43447 |
|---|---|
| 著者 | Tanaka Y, Kanai F, Tada M, Tateishi R, Sanada M, Nannya Y, Ohta M, Asaoka Y, Seto M, Shiina S, Yoshida H, Kawabe T, Yokosuka O, Ogawa S, Omata M. |
| タイトル | Gain of GRHL2 is associated with early recurrence of hepatocellular carcinoma. |
| ジャーナル | J Hepatol |
| Abstract |
BACKGROUND/AIMS:The aim of this study is to identify genomic changes that might be implicated in hepatocellular carcinoma (HCC) progression, and evaluate the associations with clinico-pathological features. METHODS:The genomic DNA of 17 hepatoma cell lines was analyzed using Affymetrix GeneChip Human Mapping 50K high-density oligonucleotide arrays. We selected representative genes from recurrent amplified regions and measured the copy number of these genes in 70 HCC clinical samples. RESULTS:We found 10 recurrent high-grade gain regions spanning less than 3 Mb in at least two hepatoma cell lines, and selected 10 representative genes. The copy number was almost normal in non-cancerous tissue and frequently amplified in Edmondson grade II or III HCC compared to Edmondson grade I HCC. Gain of TAX1BP1 in 7p15.2-1 was associated with larger tumor size and positivity of HCV antibody, and gain of CCND1 in 11q13.2-3 was associated with larger tumor size by multivariate analysis. Furthermore, a gain of GRHL2 in 8q22.3 was associated with early recurrence of HCC, controlling for clinical parameters. Decreased GRHL2 expression by RNA interference inhibits the growth of hepatoma cells, suggesting its association with cell proliferation. CONCLUSIONS:A gain of GRHL2 might be a predictive marker for HCC recurrence. |
| 巻・号 | 49(5) |
| ページ | 746-57 |
| 公開日 | 2008-11-1 |
| DOI | 10.1016/j.jhep.2008.06.019 |
| PII | S0168-8278(08)00425-X |
| PMID | 18752864 |
| MeSH | Adolescent Adult Aged Base Sequence Biomarkers, Tumor / genetics Carcinoma, Hepatocellular / genetics* Carcinoma, Hepatocellular / pathology Cell Line, Tumor Child DNA Primers / genetics DNA-Binding Proteins / genetics* Disease-Free Survival Female Gene Dosage* Humans Infant Intracellular Signaling Peptides and Proteins / genetics Liver Neoplasms / genetics* Liver Neoplasms / pathology Loss of Heterozygosity Male Middle Aged Neoplasm Proteins / genetics Oligonucleotide Array Sequence Analysis RNA Interference Recurrence Transcription Factors / genetics* Young Adult |
| IF | 20.582 |
| 引用数 | 43 |
| WOS 分野 | GASTROENTEROLOGY & HEPATOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | HuH-6(RCB1367) Hep G2 SSP-25(RCB1293) RBE(RCb1292) |