RRC ID 43473
著者 Onuki-Nagasaki R, Nagasaki A, Hakamada K, Uyeda TQ, Fujita S, Miyake M, Miyake J.
タイトル On-chip screening method for cell migration genes based on a transfection microarray.
ジャーナル Lab Chip
Abstract Cell migration plays a major role in a variety of biological processes and a detailed understanding of associated mechanisms should lead to advances in the medical sciences, for example, in drug discovery for cancer therapy. However, the traditional methods used for analysis of cell migration cannot easily be scaled up for high-throughput screening. In this study, we have attempted to develop a novel simple method for high-throughput phenotypic screening for the identification of genes that are required for cell migration. As the appropriate cell line for the method, we found NBT-L2b cells that would be suitable for screening of migration-related genes in our method without influence by other cellular processes. Moreover, the idea for printing both the labeled fibronectin, for identification of the starting region of a cell, and the green fluorescent protein (GFP) expression vector, for identification of cells that had been transfected with siRNA and of the end point of migration, brings a rapid and efficient high-throughput screening procedure. Our new method will lead to an enhanced understanding of cell migration.
巻・号 8(9)
ページ 1502-6
公開日 2008-9-1
DOI 10.1039/b803879a
PMID 18818805
MeSH Cell Line, Tumor Cell Movement / genetics* Humans Oligonucleotide Array Sequence Analysis / methods* Phenotype RNA, Small Interfering / genetics Transfection* Transgenes / genetics*
IF 6.774
引用数 25
WOS 分野 CHEMISTRY, MULTIDISCIPLINARY BIOCHEMICAL RESEARCH METHODS NANOSCIENCE & NANOTECHNOLOGY
リソース情報
ヒト・動物細胞