論文 - 詳細
| RRC ID | 43478 |
|---|---|
| 著者 | Oki M, Yamamoto H, Taniguchi H, Adachi Y, Imai K, Shinomura Y. |
| タイトル | Overexpression of the receptor tyrosine kinase EphA4 in human gastric cancers. |
| ジャーナル | World J Gastroenterol |
| Abstract |
AIM:To clarify the expression and role of Ephrin receptor A4 (EphA4) in gastric cancer in relation to clinicopathological characteristics and the expression of fibroblast growth factor receptor 1 (FGFR1) and ephrin ligands. METHODS:Eleven gastric carcinoma cell lines, 24 paired surgical fresh specimens of gastric adenocarcinoma and adjacent nontumor tissue, 74 conventional formalin-fixed, paraffin-embedded tumor specimens, and 55 specimens spotted on tissue microarray (TMA) were analyzed. Reverse transcription-PCR (RT-PCR), real-time RT-PCR, immunohistochemistry, and cell growth assays were performed. RESULTS:Overexpression of EphA4 mRNA expression was observed in 8 (73%) of 11 gastric cancer cell lines and 10 (42%) of 24 gastric cancer tissues. Overexpression of EphA4, analyzed by immunohistochemistry, was observed in 62 (48%) of 129 gastric cancer tissues. EphA4 overexpression, at the protein level, was significantly associated with depth of invasion and recurrence. EphA4 overexpression was also correlated with FGFR1 overexpression. Patients with EphA4-positive cancer had significantly shorter overall survival periods than did those with EphA4-negative cancer (P = 0.0008). The mRNAs for ephrin ligands were coexpressed in various combinations in gastric cancer cell lines and cancer tissues. Downregulation of EphA4 expression by siRNA in EphA4-overexpressing gastric cancer cell lines resulted in a significant decrease in cell growth. CONCLUSION:Our results suggest that overexpression of EphA4 plays a role in gastric cancer. |
| 巻・号 | 14(37) |
| ページ | 5650-6 |
| 公開日 | 2008-10-7 |
| DOI | 10.3748/wjg.14.5650 |
| PMID | 18837080 |
| PMC | PMC2748198 |
| MeSH | Adenocarcinoma / enzymology* Adenocarcinoma / genetics Adenocarcinoma / pathology Adenocarcinoma / therapy Cell Line, Tumor Cell Proliferation Ephrins / metabolism Gene Expression Regulation, Enzymologic Gene Expression Regulation, Neoplastic Humans Immunohistochemistry RNA Interference RNA, Messenger / metabolism RNA, Small Interfering / metabolism Receptor, EphA4 / genetics Receptor, EphA4 / metabolism* Receptor, Fibroblast Growth Factor, Type 1 / metabolism Reverse Transcriptase Polymerase Chain Reaction Stomach Neoplasms / enzymology* Stomach Neoplasms / genetics Stomach Neoplasms / pathology Stomach Neoplasms / therapy Tissue Array Analysis Treatment Outcome Up-Regulation |
| IF | 3.665 |
| 引用数 | 45 |
| WOS 分野 | GASTROENTEROLOGY & HEPATOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | |