RRC ID |
43489
|
Author |
Kazama T, Fujie M, Endo T, Kano K.
|
Title |
Mature adipocyte-derived dedifferentiated fat cells can transdifferentiate into skeletal myocytes in vitro.
|
Journal |
Biochem Biophys Res Commun
|
Abstract |
We have previously reported the establishment of preadipocyte cell lines, termed dedifferentiated fat (DFAT) cells, from mature adipocytes of various animals. DFAT cells possess long-term viability and can redifferentiate into adipocytes both in vivo and in vitro. Furthermore, DFAT cells can transdifferentiate into osteoblasts and chondrocytes under appropriate culture conditions. However, it is unclear whether DFAT cells are capable of transdifferentiating into skeletal myocytes, which is common in the mesodermal lineage. Here, we show that DFAT cells can be induced to transdifferentiate into skeletal myocytes in vitro. Myogenic induction of DFAT cells resulted in the expression of MyoD and myogenin, followed by cell fusion and formation of multinucleated cells expressing sarcomeric myosin heavy chain. These results indicate that DFAT cells derived from mature adipocytes can transdifferentiate into skeletal myocytes in vitro.
|
Volume |
377(3)
|
Pages |
780-5
|
Published |
2008-12-19
|
DOI |
10.1016/j.bbrc.2008.10.046
|
PII |
S0006-291X(08)02003-2
|
PMID |
18938140
|
MeSH |
Adipocytes / cytology*
Adipocytes / drug effects
Adipocytes / metabolism
Adipogenesis
Animals
Azacitidine / pharmacology
Base Sequence
Cell Line
Cell Lineage
Cell Transdifferentiation* / genetics
DNA Methylation / drug effects
Gene Silencing
Mice
Molecular Sequence Data
Muscle Fibers, Skeletal / cytology*
MyoD Protein / genetics
Promoter Regions, Genetic
Reverse Transcriptase Polymerase Chain Reaction
|
IF |
2.985
|
Times Cited |
63
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
Resource |
Human and Animal Cells |
10T1/2(RCB0247)
C2C12(RCB0987) |