RRC ID 43489
著者 Kazama T, Fujie M, Endo T, Kano K.
タイトル Mature adipocyte-derived dedifferentiated fat cells can transdifferentiate into skeletal myocytes in vitro.
ジャーナル Biochem Biophys Res Commun
Abstract We have previously reported the establishment of preadipocyte cell lines, termed dedifferentiated fat (DFAT) cells, from mature adipocytes of various animals. DFAT cells possess long-term viability and can redifferentiate into adipocytes both in vivo and in vitro. Furthermore, DFAT cells can transdifferentiate into osteoblasts and chondrocytes under appropriate culture conditions. However, it is unclear whether DFAT cells are capable of transdifferentiating into skeletal myocytes, which is common in the mesodermal lineage. Here, we show that DFAT cells can be induced to transdifferentiate into skeletal myocytes in vitro. Myogenic induction of DFAT cells resulted in the expression of MyoD and myogenin, followed by cell fusion and formation of multinucleated cells expressing sarcomeric myosin heavy chain. These results indicate that DFAT cells derived from mature adipocytes can transdifferentiate into skeletal myocytes in vitro.
巻・号 377(3)
ページ 780-5
公開日 2008-12-19
DOI 10.1016/j.bbrc.2008.10.046
PII S0006-291X(08)02003-2
PMID 18938140
MeSH Adipocytes / cytology* Adipocytes / drug effects Adipocytes / metabolism Adipogenesis Animals Azacitidine / pharmacology Base Sequence Cell Line Cell Lineage Cell Transdifferentiation* / genetics DNA Methylation / drug effects Gene Silencing Mice Molecular Sequence Data Muscle Fibers, Skeletal / cytology* MyoD Protein / genetics Promoter Regions, Genetic Reverse Transcriptase Polymerase Chain Reaction
IF 2.985
引用数 63
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 10T1/2(RCB0247) C2C12(RCB0987)