論文 - 詳細
| RRC ID | 43490 |
|---|---|
| 著者 | Matsuda K, Yamauchi K, Matsumoto T, Sano K, Yamaoka Y, Ota H. |
| タイトル | Quantitative analysis of the effect of Helicobacter pylori on the expressions of SOX2, CDX2, MUC2, MUC5AC, MUC6, TFF1, TFF2, and TFF3 mRNAs in human gastric carcinoma cells. |
| ジャーナル | Scand J Gastroenterol |
| Abstract |
OBJECTIVE:To investigate the phenotypic characters of carcinoma cells and the response of gastric epithelial cells to Helicobacter pylori (H. pylori) infection using the gastric carcinoma cell lines. MATERIAL AND METHODS:Real-time reverse transcription-polymerase chain reaction (RT-PCR) was used to assess the effect of H. pylori infection on mRNA levels of transcription factors (SOX2 and CDX2), mucin core proteins (MUC2, MUC5AC, and MUC6), and trefoil factor family peptides (TFF) (TFF1, TFF2, and TFF3) in gastric carcinoma cells (AGS, MKN45, and KATO III cells). H. pylori ATCC 43504 and its isogenic cag pathogenicity island (PAI) deleted mutant were used. RESULTS:These cell lines expressed mixed gastric and intestinal phenotypes. The intestinal phenotype predominated in AGS cells and gastric phenotypes in MKN45 and KATO III cells. In all three cell lines, H. pylori infection inhibited SOX2 mRNA expression, but induced the three TFFs mRNAs. In AGS cells, H. pylori induced cag PAI-dependent mRNA expression of CDX2, MUC2, MUC5AC, and MUC6. mRNA expressions of CDX2, MUC5AC, and MUC6 were inhibited in KATO III cells, whereas MUC2 mRNA expression was unchanged. In MKN45 cells, H. pylori induced the three MUCs mRNAs but inhibited CDX2 mRNA expression. CONCLUSIONS:This study provides a useful platform for selecting appropriate cell lines to model H. pylori-related changes in the gastric epithelium that mirror the changes seen in vivo. The outcome of H. pylori infection may reflect changes in the mucus gel layer caused by altered expression of mucins and TFF peptides. |
| 巻・号 | 43(1) |
| ページ | 25-33 |
| 公開日 | 2008-1-1 |
| DOI | 10.1080/00365520701579795 |
| PMID | 18938748 |
| PMC | PMC3128253 |
| MeSH | CDX2 Transcription Factor Cell Line DNA-Binding Proteins / analysis HMGB Proteins / analysis Helicobacter Infections / metabolism* Helicobacter pylori* Homeodomain Proteins / analysis Humans Mucin 5AC Mucin-2 Mucin-6 Mucins / analysis* Peptides / analysis* RNA, Messenger / analysis Reverse Transcriptase Polymerase Chain Reaction SOXB1 Transcription Factors Stomach Neoplasms / metabolism* Transcription Factors / analysis* Trefoil Factor-1 Trefoil Factor-2 Trefoil Factor-3 Tumor Cells, Cultured Tumor Suppressor Proteins / analysis |
| IF | 2.13 |
| 引用数 | 32 |
| WOS 分野 | GASTROENTEROLOGY & HEPATOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | |