RRC ID 43491
著者 Inoue N, Ikawa M, Okabe M.
タイトル Putative sperm fusion protein IZUMO and the role of N-glycosylation.
ジャーナル Biochem Biophys Res Commun
Abstract IZUMO is the mouse sperm protein proven to be essential for fusion with eggs. It contains one immunoglobulin-like domain with a conserved glycosylation site within. In the present paper, we produced transgenic mouse lines expressing unglycosylated IZUMO (N204Q-IZUMO) in Izumo1 -/- background. The expression of N204Q-IZUMO rescued the infertile phenotype of IZUMO disrupted mice, indicating glycosylation is not essential for fusion-facilitating activity of IZUMO. The N204Q-IZUMO was produced in testis in comparable amounts to wild-type IZUMO, but the amount of N204Q-IZUMO on sperm was significantly decreased by the time sperm reached the cauda epididymis. These data suggest that glycosylation is not essential for the function of IZUMO, but has a role in protecting it from fragmentation in cauda epididymis.
巻・号 377(3)
ページ 910-4
公開日 2008-12-19
DOI 10.1016/j.bbrc.2008.10.073
PII S0006-291X(08)02047-0
PMID 18952059
MeSH Amino Acid Sequence Animals Cell Fusion Dogs Epididymis / cytology Epididymis / growth & development Epididymis / metabolism Female Fertility* / genetics Glycosylation Humans Immunoglobulins / genetics Immunoglobulins / metabolism* Male Membrane Proteins / genetics Membrane Proteins / metabolism* Mice Mice, Transgenic Molecular Sequence Data Ovum / metabolism Ovum / physiology Rats Spermatozoa / metabolism Spermatozoa / physiology*
IF 2.985
引用数 45
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 CHO