論文 - 詳細
RRC ID | 43491 |
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著者 | Inoue N, Ikawa M, Okabe M. |
タイトル | Putative sperm fusion protein IZUMO and the role of N-glycosylation. |
ジャーナル | Biochem Biophys Res Commun |
Abstract |
IZUMO is the mouse sperm protein proven to be essential for fusion with eggs. It contains one immunoglobulin-like domain with a conserved glycosylation site within. In the present paper, we produced transgenic mouse lines expressing unglycosylated IZUMO (N204Q-IZUMO) in Izumo1 -/- background. The expression of N204Q-IZUMO rescued the infertile phenotype of IZUMO disrupted mice, indicating glycosylation is not essential for fusion-facilitating activity of IZUMO. The N204Q-IZUMO was produced in testis in comparable amounts to wild-type IZUMO, but the amount of N204Q-IZUMO on sperm was significantly decreased by the time sperm reached the cauda epididymis. These data suggest that glycosylation is not essential for the function of IZUMO, but has a role in protecting it from fragmentation in cauda epididymis. |
巻・号 | 377(3) |
ページ | 910-4 |
公開日 | 2008-12-19 |
DOI | 10.1016/j.bbrc.2008.10.073 |
PII | S0006-291X(08)02047-0 |
PMID | 18952059 |
MeSH | Amino Acid Sequence Animals Cell Fusion Dogs Epididymis / cytology Epididymis / growth & development Epididymis / metabolism Female Fertility* / genetics Glycosylation Humans Immunoglobulins / genetics Immunoglobulins / metabolism* Male Membrane Proteins / genetics Membrane Proteins / metabolism* Mice Mice, Transgenic Molecular Sequence Data Ovum / metabolism Ovum / physiology Rats Spermatozoa / metabolism Spermatozoa / physiology* |
IF | 2.985 |
引用数 | 45 |
WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
リソース情報 | |
ヒト・動物細胞 | CHO |