論文 - 詳細
| RRC ID | 43494 |
|---|---|
| 著者 | Arai K, Nagashima Y, Takemoto T, Nishiyama T. |
| タイトル | Mechanical strain increases expression of type XII collagen in murine osteoblastic MC3T3-E1 cells. |
| ジャーナル | Cell Struct Funct |
| Abstract |
In adult mouse, the mRNA corresponding to the alpha1 chain of type XII collagen (alpha 1(XII)) is predominantly detected in the bone. Additionally, murine osteoblastic cells, MC3T3-E1, increased the mRNA level of alpha 1(XII) response to the mechanical strain in the stretch culture system. Cyclic stretch stress resulted in a threefold increase in mRNA level of alpha 1(XII) as compared to the control experiment in MC3T3-E1. Transient transfection assays employing a reporter construct, together with site-directed mutagenesis studies, suggested that the AP-1 binding site in the first exon of mouse alpha 1(XII) gene is important for stretch stress-mediated upregulation of alpha 1(XII) expression. Electrophoretic mobility shift assay and associated antibody supershift experiments showed that stretch stress promotes the binding of c-Jun and JunD. Further chromatin immunoprecipitation experiments confirmed the participation of these transcription factors in the region. Also, the exogenous induction of the dominant negative form of c-Jun canceled the effect of stretch stress on the stimulation of the alpha 1(XII) gene. Here, we reported a potential responsive element to the stretch stress in mouse alpha 1(XII) gene. These data will provide new information on the mechanical strain-mediated transcriptional control of alpha 1(XII)-mediated fibrillogenesis in the bone. |
| 巻・号 | 33(2) |
| ページ | 203-10 |
| 公開日 | 2008-1-1 |
| DOI | 10.1247/csf.08025 |
| PII | JST.JSTAGE/csf/08025 |
| PMID | 18957791 |
| MeSH | Animals Base Sequence Bone and Bones / cytology Bone and Bones / metabolism Cell Line Cells, Cultured Collagen Type XII / genetics* Exons Mice Osteoblasts / metabolism* RNA, Messenger / genetics RNA, Messenger / metabolism Stress, Mechanical* Up-Regulation* |
| IF | 2.429 |
| 引用数 | 14 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |