論文 - 詳細
| RRC ID | 43534 |
|---|---|
| 著者 | Yagi M, Suzuki N, Takayama T, Arisue M, Kodama T, Yoda Y, Numasaki H, Otsuka K, Ito K. |
| タイトル | Lactoferrin suppress the adipogenic differentiation of MC3T3-G2/PA6 cells. |
| ジャーナル | J Oral Sci |
| Abstract |
Lactoferrin accelerates the differentiation of osteogenic and chondrogenic lineage cells, whereas it inhibits the myogenic and adipogenic differentiation of pluripotent mesenchymal cells; however, the effect of lactoferrin on the differentiation of preadipocytes is unknown. In this study, we examined the effect of lactoferrin on adipogenic differentiation using a mouse preadipocyte cell line, MC3T3-G2/PA6. The cells were cultured in differentiation medium with or without lactoferrin to induce cellular differentiation. The cell lineage was then determined by Oil Red O staining, real-time PCR screening for the mRNA expression of phenotype-specific markers, and Western blot analysis. The number of Oil Red O-positive lipid droplets decreased following treatment with lactoferrin, as did the mRNA expression of C/EBPalpha, PPARgamma, aP2, and adiponectin. Furthermore, our Western blot data revealed a decrease in PPARgamma expression attributable to lactoferrin exposure. These results suggest that lactoferrin suppresses the adipogenic differentiation of MC3T3-G2/PA6 cells. |
| 巻・号 | 50(4) |
| ページ | 419-25 |
| 公開日 | 2008-12-1 |
| DOI | 10.2334/josnusd.50.419 |
| PII | JST.JSTAGE/josnusd/50.419 |
| PMID | 19106469 |
| MeSH | 3T3 Cells Adipocytes / drug effects* Adipogenesis / drug effects Adiponectin / analysis Animals Azo Compounds Biomarkers / analysis Blotting, Western CCAAT-Enhancer-Binding Protein-alpha / analysis Cell Culture Techniques Cell Differentiation / drug effects Cell Lineage Cell Proliferation / drug effects Coloring Agents Fatty Acid-Binding Proteins / analysis Lactoferrin / pharmacology* Lipids / analysis Mice PPAR gamma / analysis Phenotype Polymerase Chain Reaction RNA, Messenger / analysis |
| IF | 1.2 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-G2/PA6(RCB1127) |