| RRC ID |
43539
|
| 著者 |
Hayashi A, Kimura M, Nakamura Y, Yasui H.
|
| タイトル |
Anti-atopic dermatitis effects and the mechanism of lactic acid bacteria isolated from Mongolian fermented milk.
|
| ジャーナル |
J Dairy Res
|
| Abstract |
We investigated the anti-allergic effects of one strain (T120) of a lactic acid bacteria (LAB) isolated from Mongolian fermented milk using atopic dermatitis (AD) model mice (NC/Nga mice). Strain T120 has already been identified as Enterococcus faecium and shown to induce strong production of IL-12 (Kimura et al. 2006). In in vitro studies, strain T120 suppressed total IgE production and induced IL-12 and IFN-gamma production by splenocytes of NC/Nga mice. The additional examination of various neutralization antibodies was performed to elucidate in detail the mechanism of depressed IgE production by strain T120. As a result, it became clear that IL-12 induced by strain T120 increased production of IFN-gamma and total IgE production was mainly controlled by the IFN-gamma. In order to define the cells which produce IL-12 powerfully by this strain, antigen-presenting cells (APCs) such as macrophages and dendritic cells (DCs) were removed from the splenocytes, and the reactivity of these cells to the strain was examined. Induction of IL-12 and IFN-gamma by strain T120 became significantly very low by removal of APCs from splenocytes. Therefore, it was clear that strain T120 acted on APCs and induced production of IL-12. Further, this strain enhanced the production of IL-10 by splenocytes. In in vivo studies, intraperitoneal injection of strain T120 inhibited serum IgE elevation and atopic dermatitis symptoms in NC/Nga mice. These results suggest that an anti-allergic effect of strain T120 depends on the increased production of IL-12 by APCs activated by the strain and following the increased production of IFN-gamma. Further, activation of regulatory T cells by strain T120 may inhibit atopic disease.
|
| 巻・号 |
76(2)
|
| ページ |
158-64
|
| 公開日 |
2009-5-1
|
| DOI |
10.1017/S0022029908003725
|
| PII |
S0022029908003725
|
| PMID |
19121229
|
| MeSH |
Animals
Cells, Cultured
Cultured Milk Products / microbiology*
Cytokines / metabolism
Dermatitis, Atopic / prevention & control*
Enterococcus faecium / physiology*
Lactic Acid / metabolism*
Male
Mice
Mice, Inbred Strains
Mongolia
Peyer's Patches / cytology
Peyer's Patches / metabolism
Specific Pathogen-Free Organisms
Spleen / cytology
Spleen / metabolism
|
| IF |
1.628
|
| 引用数 |
7
|
|
WOS 分野
|
AGRICULTURE, DAIRY & ANIMAL SCIENCE
FOOD SCIENCE & TECHNOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
