論文 - 詳細
| RRC ID | 43550 |
|---|---|
| 著者 | Nakayama H, Yaguchi T, Yoshiya S, Nishizaki T. |
| タイトル | Resveratrol induces apoptosis MH7A human rheumatoid arthritis synovial cells in a sirtuin 1-dependent manner. |
| ジャーナル | Rheumatol Int |
| Abstract |
Resveratrol, a phytoalexin, reduced the viability of MH7A cells, a human rheumatoid arthritis synovial cell line. In the apoptosis assay, resveratrol increased TUNEL-positive cells and stimulated H2A.X phosphorylation. Resveratrol disrupted mitochondrial membrane potentials in MH7A cells and stimulated cytochrome c release from the mitochondria to the cytosol. Resveratrol activated caspase-3 and caspase-9 but not caspase-8 in MH7A cells. Resveratrol upregulated the expression of the NAD-dependent deacetylase sirtuin 1 mRNA and downregulated the expression of the Bcl-X(L) mRNA, and resveratrol-induced MH7A cell death, mitochondrial damage, and caspase-3/-9 activation were prevented by sirtinol, an inhibitor of sirtuin 1. The results of the present study show that resveratrol induces MH7A cell apoptosis by activating caspase-9 and the effector caspase-3 along mitochondrial disruption as a result of reduced Bcl-X(L) expression, allowing cytochrome c release from the mitochondria into the cytosol, in a sirtuin 1-dependent manner. This suggests that resveratrol could suppress hyperplasia of synovial cells, a critical factor of rheumatoid arthritis. |
| 巻・号 | 32(1) |
| ページ | 151-7 |
| 公開日 | 2012-1-1 |
| DOI | 10.1007/s00296-010-1598-8 |
| PMID | 20697895 |
| PMC | PMC3253293 |
| MeSH | Anti-Inflammatory Agents, Non-Steroidal / pharmacology Apoptosis / drug effects* Arthritis, Rheumatoid / metabolism* Arthritis, Rheumatoid / pathology* Benzamides / pharmacology Caspase 3 / metabolism Caspase 9 / metabolism Cell Line Cytochromes c / metabolism Humans Mitochondria / metabolism Naphthols / pharmacology Resveratrol Sirtuin 1 / antagonists & inhibitors Sirtuin 1 / metabolism* Stilbenes / pharmacology* Synovial Membrane / drug effects Synovial Membrane / metabolism* Synovial Membrane / pathology* Up-Regulation / drug effects bcl-X Protein / metabolism |
| IF | 1.984 |
| 引用数 | 35 |
| WOS 分野 | RHEUMATOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MH7A(RCB1512) |