| RRC ID |
43558
|
| 著者 |
Martinez Y, Béna F, Gimelli S, Tirefort D, Dubois-Dauphin M, Krause KH, Preynat-Seauve O.
|
| タイトル |
Cellular diversity within embryonic stem cells: pluripotent clonal sublines show distinct differentiation potential.
|
| ジャーナル |
J Cell Mol Med
|
| Abstract |
Embryonic stem cells (ESC), derived from the early inner cell mass (ICM), are constituted of theoretically homogeneous pluripotent cells. Our study was designed to test this concept using experimental approaches that allowed characterization of progenies derived from single parental mouse ESC. Flow cytometry analysis showed that a fraction of ESC submitted to neural differentiation generates progenies that escape the desired phenotype. Live imaging of individual cells demonstrated significant variations in the capacity of parental ESC to generate neurons, raising the possibility of clonal diversity among ESC. To further substantiate this hypothesis, clonal sublines from ESC were generated by limit dilution. Transcriptome analysis of undifferentiated sublines showed marked differences in gene expression despite the fact that all clones expressed pluripotency markers. Sublines showed distinct differentiation potential, both in phenotypic differentiation assays and with respect to gene expression in embryoid bodies. Clones generated from another ESC line also showed individualities in their differentiation potential, demonstrating the wider applicability of these findings. Taken together, our observations demonstrate that pluripotent ESC consist of individual cell types with distinct differentiation potentials. These findings identify novel elements for the biological understanding of ESC and provide new tools with a major potential for their future in vitro and in vivo use.
|
| 巻・号 |
16(3)
|
| ページ |
456-67
|
| 公開日 |
2012-3-1
|
| DOI |
10.1111/j.1582-4934.2011.01334.x
|
| PMID |
21535399
|
| PMC |
PMC3822923
|
| MeSH |
Animals
Biomarkers / analysis
Cell Differentiation*
Cell Line
Clone Cells
Embryonic Stem Cells / cytology*
Embryonic Stem Cells / physiology
Flow Cytometry
Gene Expression Regulation, Developmental
Genetic Variation
Mice
Neurons / cytology*
Neurons / physiology
Pluripotent Stem Cells / cytology*
Pluripotent Stem Cells / physiology
Single-Cell Analysis
Transcriptome
|
| IF |
4.486
|
| 引用数 |
9
|
|
WOS 分野
|
MEDICINE, RESEARCH & EXPERIMENTAL
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-G2/PA6(RCB1127) |
